Test Code PEDBLN Pediatric Bilirbuin Panel
Specimen and Container/Tube
Specimen Type: Plasma
Preferred Specimen: Li-Heparin Mint Green Top
Other Acceptable specimens: Red Top, Gold Top, Lavendar K2 Top
Specimen Handling and Transport Instructions
UCMC Onsite Instructions:
- Collect specimen per standard collection procedure and send to laboratory immediately.
UCMC Offsite and UC MedLab Outreach Instructions:
- Samples should be centrifuged within 2 hours of collection.
- Centrifuge specimens at >2500 x g for 10 minutes at room temperature.
- Aliquot sample into plastic transport tube containing a minimum of 0.2 mL of specimen.
- Samples must be sent room temperature at 15-25oC.
Rejection Criteria
- Common specimen rejected situations include: incorrect specimen type, insufficient volume, missing or incomplete specimen identifiers, incorrect specimen transport, or specimens outside stability limits.
- Specimens that are clotted or grossly hemolyzed.
- Specimens that were transported unspun/whole blood and draw time is greater than 6 hours.
Reference Values
Test |
Age |
Sex |
Reference Range |
Total Bilirubin |
< 24 Hours |
All |
1.0 - 6.0 mg/dL |
Total Bilirubin |
24 - 48 Hours |
All |
2.0 - 8.0 mg/dL |
Total Bilirubin |
3 - 5 Days |
All |
4.0 - 12.0 mg/dL |
Total Bilirubin |
6 - 14 Days |
All |
0.6 - 7.0 mg/dL |
Test |
Age |
Sex |
Reference Range |
Bilirubin, Conjugated |
<14 Days |
All |
0.0 - 0.6 mg/dL |
Test |
Age |
Sex |
Reference Range |
Bilirubin, Unconjugated |
<24 hours |
All |
1.0 - 6.0 mg/dL |
Bilirubin, Unconjugated |
24 - 48 hours |
All |
2.0 - 8.0 mg/dL |
Bilirubin, Unconjugated |
3 - 5 Days |
All |
4.0 - 12.0 mg/dL |
Bilirubin, Unconjugated |
6 - 14 Days |
All |
0.6 - 7.0 mg/dL |
NOTE: Unconjugated Bilrubin (BU) is a calculation of Total Bilirubin (TBIL) minus the Conjugated Bilirubin (BC).
Critical Values
Analyte |
Unit |
Low |
High |
Age |
Total Bilirubin |
mg/dL |
N/A |
>18.0 |
0-14 Days |
CPT Codes
82247, 82248
LOINC Codes
1975-2, 15152-2
Volume
Preferred Volume: 0.5 mL
Minimum Volume: 0.2 mL
Specimen Stability
Storage Temperature |
Stability |
Room temperature |
1 day |
Refrigerated |
7 days |
Frozen |
6 months |
Collection Instructions
Collect specimen per standard laboratory collection procedures.
Test Components
- Total Bilirubin
- Pediatric Conjugated Bilirubin
- Calculated Unconjugated Bilirubin
Clinical Indications
Measurements of bilirubin are used in the diagnosis of liver disease, in the detection of hemolytic anemia, and to evaluate degrees of jaundice.
Methodology
Colormetric Diazo Method (Roche Diagnostics) and Endpoint Colorimetric (Modified Jendrassik-Grof) (Roche Diagnostics)
Additional Information
- Results should always be assessed in conjunction with patient's medical history, clinical examination and other findings.
- Cyanokit (hydroxocobalamin) at therapeutic concentrations causes significantly lower results. Results from certain multiple myeloma patient samples may show a positive bias in recovery. Not all multiple myeloma patients show the bias and the severity of the bias may vary between patients.
- Grossly hemolyzed specimens inhibit the diazo reaction and falsely low conjugated bilirubin results may be seen.
- Each of these test components may be ordered individually. A test or panel may be ordered only when all components are medically necessary to diagnose or treat an individual patient. If all components are not medically necessary, a less inclusive panel and/or an individual test as appropriate to treat the individual patient should be ordered.
Turnaround Time
Turnaround times are relative to the time the specimen is received in the test laboratory.
STAT Turnaround Time: 1 hour
Routine Turnaround Time: 4 hours
Testing Schedule: 24/7
Synonyms/Keyword Search
DBIL, BILD, BILC, Direct Bilirubin, NBIL
Last Review Date
8/19/2024