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The University of Chicago Medicine Laboratories

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Mayo Clinic Laboratories

Test Code PEDBC Bilirubin, Conjugated, Pediatric

Important Note

This order is for Pediatric samples ONLY.

Specimen and Container/Tube

Specimen Type: Plasma

Preferred Specimen: Li-Heparin Mint Green Top

Other Acceptable specimens: Red Top, Gold Top

Volume

Preferred Volume: 0.5 mL

Minimum Volume: 0.2 mL

Specimen Stability

Specimen Type

Storage Temperature

Stability
Serum

Room temperature

2 days

Refrigerated

7 days

Frozen (-70°C)

3 months
Plasma

Room temperature

2 days

Refrigerated

7 days

Frozen (-15 - -25°C)

6 months

Collection Instructions

Collect specimen per standard laboratory collection procedures. 

 

Specimen Handling and Transport Instructions

UCMC Onsite Instructions: 

  • Collect specimen per standard collection procedure and send to laboratory immediately. 
  • Protect specimen from light during handling and transport 

 

UCMC Offsite and UC MedLab Outreach Instructions: 

  • Samples should be centrifuged within 2 hours of collection.
  • Centrifuge specimens at >2500 x g for 10 minutes at room temperature.
  • Aliquot sample into plastic transport tube containing a minimum of 0.2 mL of specimen.
  • Samples must be sent room temperature at 15-25oC.
  • Protect specimen from light during handling and transport 

Rejection Criteria

  • Common specimen rejected situations include: incorrect specimen type, insufficient volume, missing or incomplete specimen identifiers, incorrect specimen transport, or specimens outside stability limits. 
  • Specimens that are clotted or grossly hemolyzed.
  • Specimens that were transported unspun/whole blood and draw time is greater than 6 hours.

Reference Values

Test

Age

Sex

Reference Range

Bilirubin, Conjugated

<14 Days

All

0.0 - 0.6 mg/dL

Bilirubin, Conjugated

≥14 Days ≤18 Years

All

0.0 - 0.3 mg/dL

 

Test

Age

Sex

Reference Range

Bilirubin, Unconjugated

<24 hours

All

1.0 - 6.0 mg/dL

Bilirubin, Unconjugated

24 - 48 hours

All

2.0 - 8.0 mg/dL

Bilirubin, Unconjugated

3 - 5 Days

All

4.0 - 12.0 mg/dL

Bilirubin, Unconjugated

6 - 14 Days

All

0.6 - 7.0 mg/dL

 

NOTE: Unconjugated Bilrubin (BU) is a calculation of Total Bilirubin (TBIL) minus the Conjugated Bilirubin (BC).

Critical Values

Not applicable 

Turnaround Time

Turnaround times are relative to the time the specimen is received in the test laboratory. 

 

STAT Turnaround Time: 1 hour

Routine Turnaround Time: 4 hours

Testing Schedule: 24/7

Clinical Indications

Evaluation of jaundice and liver functions.

Test Components

Conjugated Bilirubin for Pediatric Patients

Methodology

Vanadate Oxidation (Randox)

CPT Codes

82248

LOINC Codes

15152-2

Synonyms/Keyword Search

DBIL, BILD, BILC, Direct Bilirubin, PedBC, PDSTLP

Additional Information

  • Results should always be assessed in conjunction with patient's medical history, clinical examination and other findings.
  • For Non-Pediatric orders use code BC
  • Grossly hemolyzed specimens inhibit the diazo reaction and falsely low conjugated bilirubin results may be seen.

Last Review Date

6/5/2025

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