Test Code PEDBC Bilirubin, Conjugated, Pediatric
Specimen and Container/Tube
Specimen Type: Plasma
Preferred Specimen: Li-Heparin Mint Green Top
Other Acceptable specimens: Red Top, Gold Top
Specimen Stability
Specimen Type |
Storage Temperature |
Stability |
Serum |
Room temperature |
2 days |
Refrigerated |
7 days |
|
Frozen (-70°C) |
3 months |
|
Plasma |
Room temperature |
2 days |
Refrigerated |
7 days |
|
Frozen (-15 - -25°C) |
6 months |
Collection Instructions
Collect specimen per standard laboratory collection procedures.
Specimen Handling and Transport Instructions
UCMC Onsite Instructions:
- Collect specimen per standard collection procedure and send to laboratory immediately.
- Protect specimen from light during handling and transport
UCMC Offsite and UC MedLab Outreach Instructions:
- Samples should be centrifuged within 2 hours of collection.
- Centrifuge specimens at >2500 x g for 10 minutes at room temperature.
- Aliquot sample into plastic transport tube containing a minimum of 0.2 mL of specimen.
- Samples must be sent room temperature at 15-25oC.
- Protect specimen from light during handling and transport
Rejection Criteria
- Common specimen rejected situations include: incorrect specimen type, insufficient volume, missing or incomplete specimen identifiers, incorrect specimen transport, or specimens outside stability limits.
- Specimens that are clotted or grossly hemolyzed.
- Specimens that were transported unspun/whole blood and draw time is greater than 6 hours.
Reference Values
Test |
Age |
Sex |
Reference Range |
Bilirubin, Conjugated |
<14 Days |
All |
0.0 - 0.6 mg/dL |
Bilirubin, Conjugated |
≥14 Days ≤18 Years |
All |
0.0 - 0.3 mg/dL |
Test |
Age |
Sex |
Reference Range |
Bilirubin, Unconjugated |
<24 hours |
All |
1.0 - 6.0 mg/dL |
Bilirubin, Unconjugated |
24 - 48 hours |
All |
2.0 - 8.0 mg/dL |
Bilirubin, Unconjugated |
3 - 5 Days |
All |
4.0 - 12.0 mg/dL |
Bilirubin, Unconjugated |
6 - 14 Days |
All |
0.6 - 7.0 mg/dL |
NOTE: Unconjugated Bilrubin (BU) is a calculation of Total Bilirubin (TBIL) minus the Conjugated Bilirubin (BC).
Critical Values
Not applicable
Turnaround Time
Turnaround times are relative to the time the specimen is received in the test laboratory.
STAT Turnaround Time: 1 hour
Routine Turnaround Time: 4 hours
Testing Schedule: 24/7
Clinical Indications
Evaluation of jaundice and liver functions.
Test Components
Conjugated Bilirubin for Pediatric Patients
Methodology
Vanadate Oxidation (Randox)
CPT Codes
82248
LOINC Codes
15152-2
Synonyms/Keyword Search
DBIL, BILD, BILC, Direct Bilirubin, PedBC, PDSTLP
Additional Information
- Results should always be assessed in conjunction with patient's medical history, clinical examination and other findings.
- For Non-Pediatric orders use code BC
- Grossly hemolyzed specimens inhibit the diazo reaction and falsely low conjugated bilirubin results may be seen.
Last Review Date
6/5/2025
Volume
Preferred Volume: 0.5 mL
Minimum Volume: 0.2 mL