Test Code PEDBC Bilirubin, Conjugated, Pediatric
Specimen and Container/Tube
Specimen Type: Plasma
Preferred Specimen: Li-Heparin Mint Green Top
Other Acceptable specimens: Red Top, Gold Top
Specimen Stability
| Specimen Type |
Storage Temperature |
Stability |
| Serum |
Room temperature |
2 days |
|
Refrigerated |
7 days |
|
|
Frozen (-70°C) |
3 months |
|
| Plasma |
Room temperature |
2 days |
|
Refrigerated |
7 days |
|
|
Frozen (-15 - -25°C) |
6 months |
Collection Instructions
Collect specimen per standard laboratory collection procedures.
Specimen Handling and Transport Instructions
UCMC Onsite Instructions:
- Collect specimen per standard collection procedure and send to laboratory immediately.
- Protect specimen from light during handling and transport
UCMC Offsite and UC MedLab Outreach Instructions:
- Samples should be centrifuged within 2 hours of collection.
- Centrifuge specimens at >2500 x g for 10 minutes at room temperature.
- Aliquot sample into plastic transport tube containing a minimum of 0.2 mL of specimen.
- Samples must be sent room temperature at 15-25oC.
- Protect specimen from light during handling and transport
Rejection Criteria
- Common specimen rejected situations include: incorrect specimen type, insufficient volume, missing or incomplete specimen identifiers, incorrect specimen transport, or specimens outside stability limits.
- Specimens that are clotted or grossly hemolyzed.
- Specimens that were transported unspun/whole blood and draw time is greater than 6 hours.
Reference Values
|
Test |
Age |
Sex |
Reference Range |
|
Bilirubin, Conjugated |
<14 Days |
All |
0.0 - 0.6 mg/dL |
|
Bilirubin, Conjugated |
≥14 Days ≤18 Years |
All |
0.0 - 0.3 mg/dL |
|
Test |
Age |
Sex |
Reference Range |
|
Bilirubin, Unconjugated |
<24 hours |
All |
1.0 - 6.0 mg/dL |
|
Bilirubin, Unconjugated |
24 - 48 hours |
All |
2.0 - 8.0 mg/dL |
|
Bilirubin, Unconjugated |
3 - 5 Days |
All |
4.0 - 12.0 mg/dL |
|
Bilirubin, Unconjugated |
6 - 14 Days |
All |
0.6 - 7.0 mg/dL |
NOTE: Unconjugated Bilrubin (BU) is a calculation of Total Bilirubin (TBIL) minus the Conjugated Bilirubin (BC).
Turnaround Time
Turnaround times are relative to the time the specimen is received in the test laboratory.
STAT Turnaround Time: 1 hour
Routine Turnaround Time: 4 hours
Testing Schedule: 24/7
Clinical Indications
Evaluation of jaundice and liver functions.
Methodology
Vanadate Oxidation (Randox)
CPT Codes
82248
LOINC Codes
15152-2
Synonyms/Keyword Search
DBIL, BILD, BILC, Direct Bilirubin, PedBC, PDSTLP
Last Review Date
6/5/2025
Volume
Preferred Volume: 0.5 mL
Minimum Volume: 0.2 mL
Critical Values
Not applicable
Test Components
Conjugated Bilirubin for Pediatric Patients
Additional Information
- Results should always be assessed in conjunction with patient's medical history, clinical examination and other findings.
- For Non-Pediatric orders use code BC
- Grossly hemolyzed specimens inhibit the diazo reaction and falsely low conjugated bilirubin results may be seen.