Sign in →

Test Code LABRFMPOX Mpox Virus DNA, Qualitative Real-Time PCR

Important Note

Order LABRFMPOX in Epic

Specimen and Container/Tube

              Specimen Type:  Lesion
              Container/Tube
                         Preferred: Flocked swab with synthetic tip:  Rayon, Nylon, or Dacron with plastic shaft in Viral Transport                                   Media
                         Acceptable:  Nasopharygeal (NP) swab
 
               Unacceptable:  

                         Foam swabs, Cotton Swabs, Calcium alginate or swabs on wooden shaft and Dry Swab (not submitted in                                 VTM)
             
Collection Volume:  3 ml Viral Transport Media (VTM)
Minimum Collection Volume:  0.5 ml Viral Transport Media (VTM)
 

Specimen Stability

  • Room temperature: Unacceptable
    Refrigerated: 7 days
    Frozen: 30 days

Collection Instructions

    Collect in duplicate. Swab in Viral Transport Media (VTM) x 2
    Do not clean the lesion with alcohol or any other disinfectant prior to swabbing. 
 

Specimen Handling and Transport Instructions

Testing Volume:  3 ml viral transport media (VTM)
             Minimum Testing Volume:  0.5 ml viral transport media (VTM)
             Storage/Transport Temperature:  Frozen
             
             
 

Off-site:

  1. Remove swab from package.  Do not touch the tip of the swab or the length of the swab that will be in the VTM container.
  2. Unscrew the top of the collection tube.  Hold the swab in 1 hand and the collection tube in the other.
  3. Do not clean the lesion with alcohol or any other disinfectant prior to swabbing.
  4. Swab a pustule/lesion vigorously without unroofing or lancing the lesion, applying firm enough pressure so that the swab shaft, if plastic, may bend slightly.  This may result in discomfort but is necessary to obtain adequate DNA.  Swipe the swab back and forth on the lesion surface at least 2-3 times.  Then rotate and repeat on the other side of the swab.
  5. Insert the swab into the viral transport medium without touching the outside of the collection tube, and break off the end of the swab, if required.
  6. Screw the top of the collection tube back on tightly.  Leaking specimens will be rejected and not tested.
  7. Freeze samples and transport on frozen packing list.

 

On-Site:

  1. Remove swab from package.  Do not touch the tip of the swab or the length of the swab that will be in the VTM container.
  2. Unscrew the top of the collection tube.  Hold the swab in 1 hand and the collection tube in the other.
  3. Do not clean the lesion with alcohol or any other disinfectant prior to swabbing.
  4. Swab a pustule/lesion vigorously without unroofing or lancing the lesion, applying firm enough pressure so that the swab shaft, if plastic, may bend slightly.  This may result in discomfort but is necessary to obtain adequate DNA.  Swipe the swab back and forth on the lesion surface at least 2-3 times.  Then rotate and repeat on the other side of the swab.
  5. Insert the swab into the viral transport medium without touching the outside of the collection tube, and break off the end of the swab, if required.
  6. Screw the top of the collection tube back on tightly.  Leaking specimens will be rejected and not tested.
  7. Send samples to Main Lab via pneumatic tube station.


Notes:  One sample will be held frozen for 30 days in Laboratory Service Center.

Rejection Criteria

Calcium Alginate swabs • Cotton swabs • Wooden shaft swabs • Dry swabs (e.g., not submitted in VCM or equivalent)

Turnaround Time

Set up: Daily; Report available: 2-4 days

Clinical Indications

Mpox Virus DNA, Qualitative Real-Time PCR - The Mpox Virus DNA, Qualitative Real-time PCR test is intended for the qualitative detection of non-variola Orthopoxviruses and Mpox virus (clade II) DNA using swabs from human pustular or vesicular rash specimens.

Methodology

Real-Time Polymerase Chain Reaction

CPT Codes

87593 (x2)

LOINC Codes

100434-0

100888-7

31208-2

39111-0

Synonyms/Keyword Search

Monkey Pox,Orthopoxvirus,Monkeypox,Poxvirus

Volume

Collection Volume:  3 ml Viral Transport Media (VTM)
Minimum Collection Volume:  0.5 ml Viral Transport Media (VTM)


Testing Volume:  3 ml viral transport media (VTM)
             Minimum Testing Volume:  0.5 ml viral transport media (VTM)
             Storage/Transport Temperature:  Frozen