Test Code VILO Human Immunodeficiency Virus (HIV-1) Viral Load
Additional Codes
HIV-1
HIV viral load
Human Immunodeficiency Virus
HIV-1 PCR
HIV-1 Quantitation, PCR
HIV-1 RNA
Specimen and Container/Tube
Blood
Lavender-top vacutainer (EDTA) tube
Volume
Preferred Volume
Adult: 10 mL
Newborn: Obtain a minimum of 3.0 mL of newborn whole blood via venipuncture into a lavender top vacutainer (EDTA) tube. A microtainer tube does not hold a sufficient amount of blood. Cord blood and heel stick specimens cannot be used and are NOT approved for testing.
Specimen Minimum Volume
3 mL
Pediatric Volume
3 mL
Specimen Handling and Transport Instructions
See UCMC collection instructions.
Rejection Criteria
Random Collections ONLY.
NO preservatives or additives, or sample will be rejected.
Reference Values
No HIV-1 RNA detected.
Test Limitations
Detection limit is 20 IU/mL
Quantitation reportable range is 20 6,000,000 IU/mL.
Turnaround Time
Day(s) Performed
Monday – Friday
STAT DAY(S) AND TIME(S) PERFORMED
Not Available
Test Components
HIV-1 RNA quantification
Methodology
Testing is performed using FDA-approved real-time PCR assays from Roche Molecular Systems, Inc., on the Roche cobas 6800 Instrument.
CPT Codes
87536
Additional Information
Test Limitations
Detection limit is 20 IU/mL
Quantitation reportable range is 20 6,000,000 IU/mL.
Collection Instructions
Offsite Collection Instructions
See UCMC collection instructions.
UCMC Collection Instructions
Specimen Collection: Venipunctur
Specimen Processing: Spin down sample, remove plasma from cells and freeze in -20C within 24 hours of collection.
Specimen Transport: Immediate, no refrigeration.
Microbiology Specimen Collection Tables
Please see link for Microbiology Specimen Collection Tables;
- Category “Blood”, Table 1 BACTERIOLOGY, CHLAMYDIA, MYCOLOGY, MYCOBACTERIOLOGY (AFB), VIROLOGY
Critical Values
All positive culture
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LOINC Codes
30687536
Synonyms/Search Keywords
HIV-1
HIV viral load
Human Immunodeficiency Virus
HIV-1 PCR
HIV-1 Quantitation, PCR
HIV-1 RNA
Last Reviewed Date
08/16/2022