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Test Code TOBRA Tobramycin, Random

Specimen and Container/Tube

Specimen Type: Serum

Preferred Specimen: Red Top

Other Acceptable specimens: Li Heparin Mint Green Top, Lavendar K2 Top, Lavendar K3 Top

Specimen Handling and Transport Instructions

UCMC Onsite Instructions: 

Collect specimen per standard collection procedure and send to laboratory immediately. 

 

UCMC Offsite and UC MedLab Outreach Instructions: 

  • Samples should be centrifuged within 2 hours of collection.
  • Centrifuge specimens at >2500 x g for 10 minutes at room temperature.
  • Aliquot sample into plastic transport tube containing a minimum of 0.2 mL of specimen.
  • Samples must be sent refrigerated at 2-8oC.

Rejection Criteria

  • Common specimen rejected situations include: incorrect specimen type, insufficient volume, missing or incomplete specimen identifiers, incorrect specimen transport, or specimens outside stability limits. 
  • Specimens that are clotted or grossly hemolyzed.
  • Specimens that were transported unspun/whole blood and draw time is greater than 6 hours.

Reference Values

Test

Age

Sex

Reference Range

Tobramycin

All

All

N/A 

Critical Values

Analyte

Unit

Low

High

Age

Tobramycin

μg/mL

N/A

>20.0

All

CPT Codes

80200

LOINC Codes

35670-9

Volume

Preferred Volume: 0.5 mL

Minimum Volume: 0.2 mL

Specimen Stability

Storage Temperature

Stability

Room temperature

 N/A

Refrigerated

 3 days 

Frozen

 1 month

Collection Instructions

Collect specimen per standard laboratory collection procedures. 

Test Components

Tobramycin
 

Clinical Indications

Useful for monitoring adequacy of serum concentration during tobramycin therapy.

Methodology

Homogeneous Enzyme Immunoassay (Roche Diagnostics)

Additional Information

  • Results should always be assessed in conjunction with patient's medical history, clinical examination and other findings.
  • Amikacin cross-reacts with this assay. Kanamycin cross-reacts significantly; however, the assay has not been optimized to quantitate this aminoglycoside. Aminoglycosides are not generally coadministered in clinical practice, although more than one aminoglycoside may be present when switching from treatment with one to another. Samples that contain tobramycin in combination with either amikacin or kanamycin cannot be reliably quantitated by this assay.

Turnaround Time

Turnaround times are relative to the time the specimen is received in the test laboratory. 

 

STAT Turnaround Time: 1 hour

Routine Turnaround Time: 4 hours

Testing Schedule: 24/7

Synonyms/Keyword Search

N/A

Last Review Date

1/18/2023