Test Code SIRB Sirolimus
Specimen and Container/Tube
Specimen Type: Whole Blood
Preferred Specimen: EDTA Lavender Top
Other Acceptable specimens: None
Volume
Preferred Volume: 4.0 mL
Minimum Volume: 0.2 mL
Specimen Stability
Storage Temperature |
Stability |
Room temperature |
7 days |
Refrigerated |
7 days |
Frozen |
3 months |
Collection Instructions
Collect specimen per standard laboratory collection procedures immediately before scheduled dose.
Specimen Handling and Transport Instructions
UCMC Onsite Instructions:
Collect specimen per standard collection procedure immediately before scheduled dose and send to laboratory immediately.
UCMC Offsite and UC MedLab Outreach Instructions:
- Samples should be sent refrigerated.
Rejection Criteria
- Common specimen rejected situations include: incorrect specimen type, insufficient volume, missing or incomplete specimen identifiers, incorrect specimen transport, or specimens outside stability limits.
- Specimens that are clotted.
Reference Values
Test |
Age |
Sex |
Reference Range |
Sirolimus Trough |
All |
All |
4 - 20 ng/mL |
Sirolimus Peak |
All |
All |
Not applicable |
Therapeutic (reference) ranges for immunosuppressant drugs have generally not been well established, as they tend to vary depending upon type of transplant, patient clinical status and post-transplantation duration/course, concomitant use of other pharmacologic agents of the same class, the dosing/collection intervals and other known factors. The stated range is based upon commonly published guidelines, and any results are subject to physician interpretation and should be used in conjunction with other diagnostic and clinical information.
Critical Values
Not applicable
Turnaround Time
Turnaround times are relative to the time the specimen is received in the test laboratory.
STAT Turnaround Time: Not available as STAT test
Routine Turnaround Time: 4 hours
Testing Schedule: Monday – Friday (7am – 3pm), Saturday & Sunday (7am – 9am)
Clinical Indications
Monitoring whole blood sirolimus concentration during therapy.
Test Components
Sirolimus
Methodology
Liquid Chromatography/Mass Spectometry (LC-MS/MS) (Thermo Fisher Scientific)
CPT Codes
80195
LOINC Codes
TBD
Synonyms/Keyword Search
Rapamune
Additional Information
- Results should always be assessed in conjunction with patient's medical history, clinical examination and other findings.
- The recommended therapeutic range applies to trough specimens drawn immediately before a dose. Blood drawn at other times will yield higher results.
- This test was developed and its performance characteristics validated by the UCH Section of Clinical Chemistry. It has not been approved by the FDA.
- The UCH Section of Clinical Chemistry is regulated under CLIA and is qualified to perform high complexity testing. This test should only be used for clinical purposes and should not be regarded as investigational or for research.
Last Review Date
4/10/2025