Test Code PROG Progesterone
Specimen and Container/Tube
Specimen Type: Plasma
Preferred Specimen: Li-Heparin Mint Green Top
Other Acceptable specimens: Serum/Gold Top, Serum/Red Top
Volume
Preferred Volume: 0.5 mL
Minimum Volume: 0.2 mL
Specimen Stability
Storage Temperature |
Stability |
Room Temperature |
1 day |
Refrigerated |
5 days |
Frozen |
6 months Note: Freeze only once |
Collection Instructions
Collect specimen per standard laboratory collection procedures.
Specimen Handling and Transport Instructions
UCMC Onsite Instructions:
- Collect specimen per standard collection procedure and send to laboratory immediately.
UCMC Offsite and UC MedLab Outreach Instructions:
- Samples should be centrifuged within 2 hours of collection.
- Centrifuge specimens at >2500 x g for 10 minutes at room temperature.
- Aliquot sample into plastic transport tube containing a minimum of 0.2 mL of specimen.
- Samples must be sent room temperature at 20-25oC.
Rejection Criteria
- Common specimen rejected situations include: incorrect specimen type, insufficient volume, missing or incomplete specimen identifiers, incorrect specimen transport, or specimens outside stability limits.
- Specimens that are clotted or grossly hemolyzed.
- Specimens that were transported unspun/whole blood and draw time is greater than 6 hours
Reference Values
Test |
Age |
Sex |
Reference Range |
Progesterone Follicular Phase |
All |
Female |
≤0.9 ng/mL |
Progesterone Ovulation Phase |
All |
Female |
≤ 12.0 ng/mL |
Progesterone Luteal Phase |
All |
Female |
1.8 - 24 ng/mL |
Progesterone Postmenopausal |
All |
Female |
≤ 0.2 ng/mL |
Progesterone |
All |
Male |
2.0 - 6.8 mIU/mL |
Note: Reference ranges are based on in-house studies performed at the University of Chicago Medicine by the Cinical Chemistry Laboratories per the Roche cobas 8000 modular analyzer series e801 System Installation Workbook and the e801 assay product insert.
Critical Values
Not applicable
Turnaround Time
Turnaround times are relative to the time the specimen is received in the test laboratory.
STAT Turnaround Time: 1 hour
Routine Turnaround Time: 4 hours
Testing Schedule: 24/7
Clinical Indications
The etermination of progesterone is utilized in fertility diagnosis for the detection of ovulation and assessment of the luteal phase. The progesterone concentration correlates with the development and regression of the corpus luteum.
Test Components
Progesterone
Methodology
Electrochemiluminescence immunoassay (ECLIA) (Roche Diagnostics)
CPT Codes
84144
LOINC Codes
TBD
Synonyms/Keyword Search
Progesterone
Additional Information
This test may be affected by biotin interference. Patients on high dose biotin (5mg/day or more) should not have samples drawn until at least 24 hours after the last biotin administration.
Phenylbutazone at therapeutic dosage levels showed interference with the assay (progesterone values decreased).
Last Review Date
4/10/2025