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The University of Chicago Medicine Laboratories

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Specimen Collection and Handling

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Mayo Clinic Laboratories

Test Code PROG Progesterone

Specimen and Container/Tube

Specimen Type: Plasma

Preferred Specimen: Li-Heparin Mint Green Top

Other Acceptable specimens: Serum/Gold Top, Serum/Red Top

Volume

Preferred Volume: 0.5 mL

Minimum Volume: 0.2 mL

Specimen Stability

Storage Temperature

Stability

Room Temperature

1 day

Refrigerated

5 days

Frozen

6 months

Note: Freeze only once

 

Specimen Handling and Transport Instructions

UCMC Onsite Instructions: 

  • Collect specimen per standard collection procedure and send to laboratory immediately. 

 

UCMC Offsite and UC MedLab Outreach Instructions: 

  • Samples should be centrifuged within 2 hours of collection.
  • Centrifuge specimens at >2500 x g for 10 minutes at room temperature.
  • Aliquot sample into plastic transport tube containing a minimum of 0.2 mL of specimen.
  • Samples must be sent room temperature at 20-25oC.

Rejection Criteria

  • Common specimen rejected situations include: incorrect specimen type, insufficient volume, missing or incomplete specimen identifiers, incorrect specimen transport, or specimens outside stability limits. 
  • Specimens that are clotted or grossly hemolyzed.
  • Specimens that were transported unspun/whole blood and draw time is greater than 6 hours

Reference Values

Test

Age

Sex

Reference Range

Progesterone

Follicular Phase

All

Female

≤0.9 ng/mL

Progesterone

Ovulation Phase

All

Female

≤ 12.0 ng/mL 

Progesterone

Luteal Phase

All

Female

1.8 - 24 ng/mL

Progesterone Postmenopausal

All

Female

≤ 0.2 ng/mL

Progesterone

All

Male

2.0 - 6.8 mIU/mL

Note: Reference ranges are based on in-house studies performed at the University of Chicago Medicine by the Cinical Chemistry Laboratories per the Roche cobas 8000 modular analyzer series e801 System Installation Workbook and the e801 assay product insert. 

Clinical Indications

The etermination of progesterone is utilized in fertility diagnosis for the detection of ovulation and assessment of the luteal phase. The progesterone concentration correlates with the development and regression of the corpus luteum.

Test Components

Progesterone

Methodology

Electrochemiluminescence immunoassay (ECLIA) (Roche Diagnostics)

CPT Codes

84144

Additional Information

This test may be affected by biotin interference. Patients on high dose biotin (5mg/day or more) should not have samples drawn until at least 24 hours after the last biotin administration.

 

Phenylbutazone at therapeutic dosage levels showed interference with the assay (progesterone values decreased).

Last Review Date

4/10/2025

Critical Values

Not applicable

LOINC Codes

TBD

Collection Instructions

Collect specimen per standard laboratory collection procedures. 

 

Turnaround Time

Turnaround times are relative to the time the specimen is received in the test laboratory. 

 

STAT Turnaround Time: 1 hour

Routine Turnaround Time: 4 hours

Testing Schedule:  24/7

Synonyms/Keyword Search

Progesterone