Test Code BC Bilirubin, Conjugated
Specimen and Container/Tube
Specimen Type: Plasma
Preferred Specimen: Li-Heparin Mint Green Top
Other Acceptable specimens: Serum/Red Top, Serum/Gold Top
Collection Instructions
Collect specimen per standard laboratory collection procedures.
Specimen Handling and Transport Instructions
UCMC Onsite Instructions:
- Collect specimen per standard collection procedure and send to laboratory immediately.
- Protect specimen from light during handling and transport
UCMC Offsite and UC MedLab Outreach Instructions:
- Samples should be centrifuged within 2 hours of collection.
- Centrifuge specimens at >2500 x g for 10 minutes at room temperature.
- Aliquot sample into plastic transport tube containing a minimum of 0.2 mL of specimen.
- Samples must be sent room temperature at 15-25oC.
- Protect specimen from light during handling and transport
Reference Values
Test |
Age |
Sex |
Reference Range |
Bilirubin, Conjugated |
<14 Days |
All |
0.0 - 0.6 mg/dL |
Bilirubin, Conjugated |
>14 Days |
All |
0.0 - 0.3 mg/dL |
Test |
Age |
Sex |
Reference Range |
Bilirubin, Unconjugated |
<24 hours |
All |
1.0 - 6.0 mg/dL |
Bilirubin, Unconjugated |
24 - 48 hours |
All |
2.0 - 8.0 mg/dL |
Bilirubin, Unconjugated |
3 - 5 Days |
All |
4.0 - 12.0 mg/dL |
Bilirubin, Unconjugated |
6 - 14 Days |
All |
0.6 - 7.0 mg/dL |
Bilirubin, Unconjugated |
>14 Days |
All |
0.1 - 1.0 mg/dL |
NOTE: Unconjugated Bilrubin (BU) is a calculation of Total Bilirubin (TBIL) minus the Conjugated Bilirubin (BC).
Volume
Preferred Volume: 0.5 mL
Minimum Volume: 0.2 mL
Specimen Stability
Storage Temperature |
Stability |
Room temperature |
2 days |
Refrigerated |
7 days |
Frozen |
6 months |
Rejection Criteria
- Common specimen rejected situations include: incorrect specimen type, insufficient volume, missing or incomplete specimen identifiers, incorrect specimen transport, or specimens outside stability limits.
- Specimens that are clotted or grossly hemolyzed.
- Specimens that were transported unspun/whole blood and draw time is greater than 6 hours.
Critical Values
Not applicable
Test Components
Conjugated Bilirubin
Clinical Indications
Evaluation of jaundice and liver functions.
Methodology
Endpoint Colorimetric (Modified Jendrassik-Grof) (Roche Diagnostics)
CPT Codes
82248
LOINC Codes
15152-2
Additional Information
- Results should always be assessed in conjunction with patient's medical history, clinical examination and other findings.
- Also included in Bilirubin Panel; BILN order code
- For Pediatric orders use code PEDBC
- Grossly hemolyzed specimens inhibit the diazo reaction and falsely low conjugated bilirubin results may be seen.
Turnaround Time
Turnaround times are relative to the time the specimen is received in the test laboratory.
STAT Turnaround Time: 1 hour
Routine Turnaround Time: 4 hours
Testing Schedule: 24/7
Synonyms/Keyword Search
DBIL, BILD, BILC, Direct Bilirubin
Last Review Date
6/5/2025