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HelpTest Code LABMDONCOSCRNCYT OncoScreen FFPE/Cytology
Specimen and Container/Tube
Specimen Type: Tumor Tissue
Preferred Specimen: FFPE Slides or Cytology Smear
Other Acceptable Specimens: not applicable
Appropriate specimens should contain >20% tumor cells and enough total cells to produce adequate DNA yield (typically >50,000 total cells (FFPE) or >2000 total cells (cytology smear)). Specimens with less than 20% tumor cells may be tested at the discretion of the attending molecular pathologist.
Specimen Stability
n/a
Collection Instructions
Collect specimen per standard laboratory collection procedures.
Specimen Handling and Transport Instructions
UCM Onsite Instructions: Collect specimen per standard collection procedured and send to the laboratory immediately.
Clinical Indications
Targeted and rapid assay covering hotspot regions in 50 cancer associated genes for genomic profiling of solid tumor and hematological malignancy specimens to determine appropriate therapies. It also can be useful for diagnosis, prognosis, clinical trials selection and for translational research.
CPT Codes
81445
88381
Methodology
Next generation sequencing
Additional Information
Detects hotspot regions in 50 genes that are commonly mutated in cancer: ABL1, AKT1, ALK, APC, ATM, BRAF, CDH1, CDKN2A, CSF1R, CTNNB1, EGFR, ERBB2, ERBB4, EZH2, FBXW7, FGFR1, FGFR2, FGFR3, FLT3, GNA11, GNAQ, GNAS, HNF1A, HRAS, IDH1, IDH2, JAK2, JAK3, KDR, KIT, KRAS, MET, MLH1, MPL, NOTCH1, NPM1, NRAS, PDGFRA, PIK3CA, PTEN, PTPN11, RB1, RET, SMAD4, SMARCB1, SMO, SRC, STK11, TP53, VHL. Will not detect mutations below the analytical sensitivity of the assay (5% variant allelic frequency).
Ordering Notes
For questions about Molecular Pathology orders, email MolecularDxLab@uchicagomedicine.org
Volume
Preferred Volume: >10 slides (FFPE), 1 slide (Cytology smear)
Minimum Volume: 10 slides (FFPE), 1 slide (Cytology Smear)
Unstained slides containing FFPE tissue are acceptable. At least 10 microtome sections of 5 micron thickness should be submitted for testing. One adequate cytology smear should be submitted for testing.
Turnaround Time
Turnaround times are relative to the time the specimen is received in the testing laboratory section.
STAT Turnaround Time: n/a
Routine Turnaround Time: ≤10 days
Testing Schedule: M-F 7:00am-5:00pm
Infectious Specimen
N/A
Search Keywords
Hotspot panel, cancer, mutation, NGS, NextGen, Sequencing, Gene