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The University of Chicago Medicine Laboratories

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Mayo Clinic Laboratories

Test Code HSVPCR Herpes Simplex Virus (HSV), Qualitative PCR, non-CSF

Important Note

This test was developed and its performance characteristics determined by the University of Chicago Medical Center Clinical Microbiology Lab. It has not been cleared or approved by the U.S. Food and Drug Administration. The FDA does not require this test to go through premarket FDA review.

This laboratory is certified under the Clinical Laboratory Improvement Amendments (CLIA) as qualified to perform high complexity laboratory testing. This test is used for clinical purposes. It should not be regarded as investigational or research.

Infectious

Additional Codes

LABHSVP

Specimen and Container/Tube

Specimen Type: Herpetic cutaneous and mucocutaneous lesions

Preferred Specimen: Universal Transport Medium (UTM)

Specimen Stability

Storage Temperature

Stability

Room temperature

(20 - 25°C)

not acceptable

Refrigerated

(2 - 10°C)

 7 days

Frozen

(-20°C)

not acceptable

Collection Instructions

Specimen Collection: Disrupt surface of lesion and collect fluid with swab. Place swab into UTM.

Specimen Transport: Immediate

Specimen Handling and Transport Instructions

UCMC Onsite Instructions: Collect specimen per standard collection procedure and send to laboratory immediately. 

 

UCMC Offsite and UC MedLab Outreach Instructions: Collect specimen per standard collection procedure and send to laboratory immediately. Specimens must be received within 2 hours. If transport is delayed, refrigerate specimens prior to transport.

Rejection Criteria

  • Common specimen rejected situations include: incorrect specimen type, insufficient volume, incorrect collection container, missing or incomplete specimen identifiers, incorrect specimen transport, or specimens outside stability limits. 
  • Specimens collected with calcium alginate swabs will be rejected.

Reference Values

 

Analyte

Age

Sex

Reference Range

Negative

All

All

No HSV DNA detected

Turnaround Time

Turnaround times are relative to the time the specimen is received in the test laboratory. 

STAT Turnaround Time: not available

Routine Turnaround Time: 24 hours

Testing Schedule: Monday - Sunday

Clinical Indications

  • Aid in the differential diagnosis of HSV-1 and HSV-2 infections in patients with signs and symptoms of HSV-1 or HSV-2 infection.
  • The assay is not intended for use as a screening test for the presence of HSV-1 and HSV-2 in blood or blood products.

Methodology

Diasorin Simplexa® automated PCR

CPT Codes

87529

LOINC Codes

30687529

Synonyms/Keyword Search

HSV, Herpes, Herpes PCR, Herpes DNA, Herpes Simplex Virus detection

Last Review Date

09/23/2025