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Test Code HSVPCR Herpes Simplex Virus (HSV), Qualitative PCR, non-CSF

Important Note

Real-Time Polymerase chain reaction/DNA Probe Hybridization by LightCycler
This test was developed and its performance characteristics determined by the University of Chicago Medical Center Clinical Microbiology Lab. It has not been cleared or approved by the U.S. Food and Drug Administration. The FDA does not require this test to go through premarket FDA review. This laboratory is certified under the Clinical Laboratory Improvement Amendments (CLIA) as qualified to perform high complexity laboratory testing. This test is used for clinical purposes. It should not be regarded as investigational or research.

Additional Codes

HSV
Herpes
Herpes PCR
Herpes DNA
Herpes Simplex Virus detection

Specimen and Container/Tube

Lesion (from genital, oral, ocular (eye), dermal (skin)), Tissue, Surface swab (newborn only), BAL

Viral transport medium.  Sterile container preferred for BAL

Specimen Handling and Transport Instructions

See UCMC collection instructions.

Rejection Criteria

Whole blood/unspun samples greater than 6 hours will be rejected.

Reference Values

No HSV (1 or 2) DNA detected

Test Limitations: Detection limit: 500 copies/mL

Critical Values

HSV (1 or 2) DNA detected

All positive  culture

 

 

 

 

All

Turnaround Time

Day(s) Performed

Monday through Friday

STAT DAY(S) AND TIME(S) PERFORMED

Not Available

CPT Codes

87529

LOINC Codes

30687529

Collection Instructions

Offsite Collection Instructions

See UCMC collection instructions.

UCMC Collection Instructions

Specimen Source: Lesion
Specimen Collection: Clean overlying skin surface with 70% alcohol. Aspirate with syringe, curette the lining of the lesion, or obtain tissue biopsy.
Specimen Transport: <2 hr ambient, 7 days refrigerated
 

Specimen Source: Tissue
Specimen Collection: Surgically obtained
Specimen Transport: <2 hr ambient, 7 days refrigerated
 

Specimen Source: Broncho-alveolar Lavage (BAL)
Specimen Collection: Aspirate through inner chamber at bronchoscopy
Specimen Transport: <2 hr ambient, 7 days refrigerated
 

Microbiology Specimen Collection Tables
Please see link  for Microbiology Specimen Collection Tables;

  • See appropriate category in Table 1 BACTERIOLOGY, CHLAMYDIA, MYCOLOGY, MYCOBACTERIOLOGY (AFB), VIROLOGY

Test Components

Lesion [from genital, oral, ocular (eye), dermal (skin)], Tissue, Surface swab (newborn only), BAL

Methodology

Real-Time Polymerase chain reaction/DNA Probe Hybridization by LightCycler
This test was developed and its performance characteristics determined by the University of Chicago Medical Center Clinical Microbiology Lab. It has not been cleared or approved by the U.S. Food and Drug Administration. The FDA does not require this test to go through premarket FDA review. This laboratory is certified under the Clinical Laboratory Improvement Amendments (CLIA) as qualified to perform high complexity laboratory testing. This test is used for clinical purposes. It should not be regarded as investigational or research.

Synonyms/Search Keywords

HSV
Herpes
Herpes PCR
Herpes DNA
Herpes Simplex Virus detection

Last Reviewed Date

08/15/2022

Additional Information

Real-Time Polymerase chain reaction/DNA Probe Hybridization by LightCycler
This test was developed and its performance characteristics determined by the University of Chicago Medical Center Clinical Microbiology Lab. It has not been cleared or approved by the U.S. Food and Drug Administration. The FDA does not require this test to go through premarket FDA review. This laboratory is certified under the Clinical Laboratory Improvement Amendments (CLIA) as qualified to perform high complexity laboratory testing. This test is used for clinical purposes. It should not be regarded as investigational or research.