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Test Code HBVL Hepatitis B Virus (HBV) Quantitative PCR, Blood

Infectious

Specimen and Container/Tube

Specimen Type: Whole blood

Preferred Specimen: Lavender EDTA tube

Volume

Preferred Volume: 5 - 10 mL

Minimum Volume: 3 mL

Specimen Stability

Storage Temperature

Stability

Room temperature

(20 - 25°C)

24 hours

Refrigerated

(2 - 10°C)

 24 hours

Frozen

(-20°C)

not acceptable

Collection Instructions

Specimen Collection: Venipucture


Specimen Transport: Immediate

Specimen Handling and Transport Instructions

UCMC Onsite Instructions: Collect specimen per standard collection procedure and send to laboratory immediately. 

 

UCMC Offsite and UC MedLab Outreach Instructions: Collect specimen per standard collection procedure and send to laboratory immediately. If transport is delayed, refrigerate specimens prior to transport.

Rejection Criteria

  • Common specimen rejected situations include: incorrect specimen type, insufficient volume, incorrect collection container, missing or incomplete specimen identifiers, incorrect specimen transport, or specimens outside stability limits. 
  • Only one test request per day is accepted.
  • Whole blood/unspun samples greater than 24 hours will be rejected.
  • Whole blood not collected in Lavender top vacuatainer tube will be rejected.

Reference Values

 

Analyte

Age

Sex

Reference Range

Negative

All

All

No HBV DNA detected

Detection limit

All

All

 6.6 IU/mL

Quantitation Reportable Range

All

All

 10 - 100,000,000 IU/mL

Quantitation Reportable Range Log

All

All

 1.00 - 8.00 IU/mL
 

Turnaround Time

Turnaround times are relative to the time the specimen is received in the test laboratory. 

STAT Turnaround Time: not available

Routine Turnaround Time: 72 hours

Testing Schedule: Monday - Sunday

Clinical Indications

  • cobas® HBV is not intended for use as a screening test for the presence of HBV in blood or blood products or as a diagnostic test to confirm the presence of HBV infection.

  • Quantitation of HBV DNA is dependent on the number of virus particles present in the samples and may be affected by sample collection methods, patient factors (i.e., age, presence of symptoms), and/or stage of infection.

CPT Codes

87517

LOINC Codes

30687517

Methodology

Roche cobas® 6800 automated PCR

Synonyms/Keyword Search

HEP B VIRAL LOAD, HBV, HEPATITIS B LEVELS, HBVL, HEPATITIS B DNA PCR, HEPATITIS B PCR, HEPATITIS B VIRAL LOAD

Last Review Date

09/05/2025