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Test Code E2 Estradiol

Important Note

This test may be affected by biotin interference. Patients on high dose biotin (5mg/day or more) should not have samples drawn until at least 24 hours after the last biotin administration.

Specimen and Container/Tube

Specimen Type: Plasma

Preferred Specimen: Li-Heparin Mint Green Top

Other Acceptable specimens: Serum/Red Top, Serum/Gold Top, Plasma/Lavendar K2 Top, Plasma/Lavendar K3 Top

Specimen Handling and Transport Instructions

UCMC Onsite Instructions: 

Collect specimen per standard collection procedure and send to laboratory immediately. 

 

UCMC Offsite and UC MedLab Outreach Instructions: 

  • Samples should be centrifuged within 2 hours of collection.
  • Centrifuge specimens at >2500 x g for 10 minutes at room temperature.
  • Aliquot sample into plastic transport tube containing a minimum of 0.2 mL of specimen.
  • Samples must be sent room temperature at 20-25oC.

 

Rejection Criteria

  • Common specimen rejected situations include: incorrect specimen type, insufficient volume, missing or incomplete specimen identifiers, incorrect specimen transport, or specimens outside stability limits. 
  • Specimens that are clotted or grossly hemolyzed.
  • Specimens that were transported unspun/whole blood and draw time is greater than 6 hours

Reference Values

 

Test

Age

Sex

Reference Range

E2  Follicular phase

All

Female

12 - 233 pg/mL

E2 Ovulation phase

All

Female

41 - 398 pg/mL

E2 Luteal phase

All

Female

22 - 341 pg/mL

E2 Postmenopausal*

All

Female

 ≤ 25 pg/mL

E2

18 - 35

Males

11 - 43 pg/mL

NOTE: Reference: Roche Package Insert, Estradiol III, ms_06656021190V4.0

*Per communcation with Dr. Baumann, based on reference validation study done at Mayo 

Critical Values

Analyte

Unit

Low

High

Age

N/A

N/A

N/A

N/A

N/A

CPT Codes

82670

LOINC Codes

35384-7

Volume

Preferred Volume: 0.5 mL

Minimum Volume: 0.2 mL

Specimen Stability

Storage Temperature

Stability

Room temperature

24 hours

Refrigerated

2 days

Frozen

6 months

Collection Instructions

Collect specimen per standard laboratory collection procedures. 

 

Test Components

Estradiol

Clinical Indications

Estrogens are responsible for the development of the secondary female sex characteristics. Together with gestagens they control all the important female reproductive process.

Methodology

Electrochemiluminescence immunoassay (ECLIA) - Roche Diagnostics

Additional Information

  • Results should always be assessed in conjunction with patient's medical history, clinical examination and other findings.
  • The determination of estradiol is utilized clinically in the elucidation of fertility disorders in the hypothalamus-pituitary-gonad axis, gynecomastia, estrogen-producing ovarian and testicular tumors.
  • Further clinical indications are the monitoring of fertility therapy and determining the time ovulation within the framework of in vitro fertilization (IVF).
  • Steroid drugs may interfere with this test.

Turnaround Time

Turnaround times are relative to the time the specimen is received in the test laboratory. 

 

STAT Turnaround Time: Not Available as STAT test

Routine Turnaround Time: up to 4 hours

Testing Schedule:  24/7

Synonyms/Keyword Search

17β-Estradiol, Estrogen, E2

Last Review Date

1/26/2023

 

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