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Test Code DOH Vitamin D, 25-hydroxyvitamin

Specimen and Container/Tube

Specimen Type: Serum

Preferred Specimen: Red Top

Other Acceptable specimens (Add On Orders ONLY): Serum/Gold Top, Li-Heparin Plasma/Mint Top

Specimen Handling and Transport Instructions

UCMC Onsite Instructions: 

Collect specimen per standard collection procedure and send to laboratory immediately. 

 

UCMC Offsite and UC MedLab Outreach Instructions: 

  • Samples should be centrifuged within 2 hours of collection.
  • Centrifuge specimens at >2500 x g for 10 minutes at room temperature.
  • Aliquot sample into plastic transport tube containing a minimum of 0.2 mL of specimen.
  • Samples must be sent refrigerated at 2-8oC.

 

Rejection Criteria

  • Common specimen rejected situations include: incorrect specimen type, insufficient volume, missing or incomplete specimen identifiers, incorrect specimen transport, or specimens outside stability limits. 
  • Specimens that are clotted or grossly hemolyzed.
  • Specimens that were transported unspun/whole blood and draw time is greater than 6 hours

Reference Values

 

Test

Age

Sex

Reference Range

DOH Deficiency

All

All

<12 ng/mL

DOH Potential Deficiency

All

All

12 - 19 ng/mL

DOH Potential Insufficiency

All

All

20 - 29 ng/mL

DOH Optimum Concentration

All

All

≥ 30 ng/mL

Critical Values

Analyte

Unit

Low

High

Age

N/A

N/A

N/A

N/A

N/A

CPT Codes

82306

LOINC Codes

62292-8

Volume

Preferred Volume: 0.5 mL

Minimum Volume: 0.2 mL

Specimen Stability

Storage Temperature

Stability

Room temperature

4 days

Refrigerated

8 hours

Frozen

24 weeks

Collection Instructions

Collect specimen per standard laboratory collection procedures. 

 

Test Components

Total 25-hydroxyvitamin D

Clinical Indications

Used in the assessment of vitamin D sufficiency in adults.

Methodology

Electrochemiluminescence immunoassay (ECLIA) - Roche Diagnostics

Additional Information

  • Results should always be assessed in conjunction with patient's medical history, clinical examination and other findings.
  • Vitamin D is essential for bone health. In children, severe deficiency leads to bone-malformation, known as rickets. Milder degrees of insufficiency are believed to cause reduced efficiency in utilization of dietary calcium.
  • Vitamin D deficiency causes muscle weakness; in elderly the risk of falling has been attributed to the effect of vitamin D on muscle function.
  • Vitamin D deficiency is a common cause of secondary hyperparathyroidism. Elevations of parathyroid hormone levels, especially in elderly vitamin D deficient adults can result in osteomalacia, increased bone turnover, reduced bone mass and risk of bone fractures.
  • Low 25-hydroxyvitamin D concentrations are also associated with lower bone mineral density.

Turnaround Time

Turnaround times are relative to the time the specimen is received in the test laboratory. 

 

STAT Turnaround Time: Not Available as STAT test

Routine Turnaround Time: up to 4 hours

Testing Schedule:  24/7

Synonyms/Keyword Search

Total 25-hydroxyvitamin D, 25-Hydroxy Vitamin D, 25-OH Vitamin D

Last Review Date

6/7/2024