Test Code CORVP Respiratory Panel PCR (NAAT)
Infectious
Specimen and Container/Tube
Specimen Type: Nasopharyngeal aspirate, nasopharyngeal swab
Preferred Specimen: Universal Transport Medium (UTM)
Specimen Stability
|
Storage Temperature |
Stability |
|
Room temperature (20 - 25°C) |
4 hours |
|
Refrigerated (2 - 10°C) |
3 days |
|
Frozen (-20°C) |
30 days |
Collection Instructions
Specimen Collection:
Nasopharyngeal swab:
- Tilt the patient’s head back 70 degrees. Insert the flocked swab through the nostril parallel to the palate (not upwards) until resistance is encountered and the swab reaches a depth equal to the distance from the nostrils to the outer opening of the ear.
- Rotate the flocked swab 2-3 times and hold the swab in place for 5-10 seconds to ensure maximum absorbency. Specimens can be collected from both sides using the same swab, but it is not necessary to collect specimens from both sides if the minitip is saturated with fluid from the first collection.
- Put the swab in the viral transport media and break the shaft at the designated breakpoint. Tightly close the lid.
Nasopharyngeal aspirate:
- Discard liquid from 0.9% NaCl syringe, leaving 1 mL in the syringe.
- Measure length of butterfly tubing needed by holding next to nose with ends at top and bottom of nose.
- Cut butterfly tubing, discard needle end in sharps container.
- Attach tubing to the syringe.
- Recline patient to a 45* angle; insert tubing.
- Quickly instill the 1 mL of saline and then aspirate back into syringe.
- Transfer aspirate into viral transport media (wash media in and out of syringe to instill maximum specimen).
- Tightly close lid.
Specimen Transport: Immediate
Specimen Handling and Transport Instructions
UCMC Onsite Instructions: Collect specimen per standard collection procedure and send to laboratory immediately.
UCMC Offsite and UC MedLab Outreach Instructions: Collect specimen per standard collection procedure and send to laboratory immediately. If transport is delayed, refrigerate specimens prior to transport, if applicable to specimen type.
Rejection Criteria
- Common specimen rejected situations include: incorrect specimen type, insufficient volume, incorrect collection container, missing or incomplete specimen identifiers, incorrect specimen transport, or specimens outside stability limits.
- Negative results in the setting of a respiratory illness may be due to infection with pathogens that are not detected by this test, or lower respiratory tract infection that may not be detected by an NPS specimen. Positive results do not rule out coinfection with other organisms. The agent(s) detected by the BIOFIRE RP2.1 may not be the definite cause of disease. Additional laboratory testing (e.g. bacterial and viral culture, immunofluorescence, and radiography) may be necessary when evaluating a patient with possible respiratory tract infection.
Reference Values
|
Analyte |
Age |
Sex |
Reference Range |
|
SARS-CoV-2 (COVID-19) |
All |
All |
Not Detected |
|
Influenza A, Influenza A subtypes H1,H3, and H1-2009 |
All |
All |
Not Detected |
|
Influenza B |
All |
All |
Not Detected |
|
Respiratory Syncytial Virus (RSV) |
All |
All |
Not Detected |
|
Parainfluenza viruses type 1-4 |
All |
All |
Not Detected |
|
Adenovirus |
All |
All |
Not Detected |
|
Metapneumovirus |
All |
All |
Not Detected |
|
Coronavirus HKU1 |
All |
All |
Not Detected |
|
Coronavirus NL63 |
All |
All |
Not Detected |
|
Coronavirus 229E |
All |
All |
Not Detected |
|
Coronavirus OC43 |
All |
All |
Not Detected |
|
Enterovirus/Rhinovirus |
All |
All |
Not Detected |
|
Mycoplasma pneumoniae |
All |
All |
Not Detected |
|
Chlamydophila pneumoniae |
All |
All |
Not Detected |
|
Bordetella pertussis |
All |
All |
Not Detected |
|
Bordetella parapertussis |
All |
All |
Not Detected |
Critical Values
|
Analyte |
Result |
| Bordetella pertussis |
Detected |
Turnaround Time
Turnaround times are relative to the time the specimen is received in the test laboratory.
STAT Turnaround Time: not available
Routine Turnaround Time: 3 hours
Testing Schedule: Monday - Sunday
Clinical Indications
- Simultaneous qualitative detection and identification of multiple respiratory viral and bacterial nucleic acids in nasopharyngeal swabs (NPS) obtained from individuals suspected of respiratory tract infections, including COVID-19.
CPT Codes
300002020 - 0202U, 30687633, 30587798 × 4
LOINC Codes
30687176, 30587205
Methodology
BioFire® automated Multiplex Polymerase Chain Reaction (PCR)
Synonyms/Keyword Search
Respiratory Viral Panel, Respiratory Panel, SARS-CoV-2, COVID-19, Influenza A, Influenza B, Respiratory Syncytial Virus (RSV), Parainfluenza, Adenovirus, Metapneumovirus, Coronavirus, Enterovirus, Rhinovirus, Mycoplasma pneumoniae, Chlamydophila pneumoniae, Bordetella pertussis, Bordetella parapertussis, Flu
Last Review Date
09/27/2025