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Test Code CORVP Respiratory Panel PCR (NAAT)

Infectious

Specimen and Container/Tube

Specimen Type: Nasopharyngeal aspirate, nasopharyngeal swab

Preferred Specimen: Universal Transport Medium (UTM)

Specimen Stability

Storage Temperature

Stability

Room temperature

(20 - 25°C)

4 hours

Refrigerated

(2 - 10°C)

3 days

Frozen

(-20°C)

30 days

Collection Instructions

Specimen Collection: 

 

Nasopharyngeal swab:

  1. Tilt the patient’s head back 70 degrees. Insert the flocked swab through the nostril parallel to the palate (not upwards) until resistance is encountered and the swab reaches a depth equal to the distance from the nostrils to the outer opening of the ear.
  2. Rotate the flocked swab 2-3 times and hold the swab in place for 5-10 seconds to ensure maximum absorbency. Specimens can be collected from both sides using the same swab, but it is not necessary to collect specimens from both sides if the minitip is saturated with fluid from the first collection.
  3. Put the swab in the viral transport media and break the shaft at the designated breakpoint. Tightly close the lid.

 
Nasopharyngeal aspirate:

  1. Discard liquid from 0.9% NaCl syringe, leaving 1 mL in the syringe.
  2. Measure length of butterfly tubing needed by holding next to nose with ends at top and bottom of nose.
  3. Cut butterfly tubing, discard needle end in sharps container.
  4. Attach tubing to the syringe.
  5. Recline patient to a 45* angle; insert tubing.
  6. Quickly instill the 1 mL of saline and then aspirate back into syringe.
  7. Transfer aspirate into viral transport media (wash media in and out of syringe to instill maximum specimen).
  8. Tightly close lid.


Specimen Transport: Immediate

Specimen Handling and Transport Instructions

UCMC Onsite Instructions: Collect specimen per standard collection procedure and send to laboratory immediately. 

 

UCMC Offsite and UC MedLab Outreach Instructions: Collect specimen per standard collection procedure and send to laboratory immediately. If transport is delayed, refrigerate specimens prior to transport, if applicable to specimen type.

Rejection Criteria

  • Common specimen rejected situations include: incorrect specimen type, insufficient volume, incorrect collection container, missing or incomplete specimen identifiers, incorrect specimen transport, or specimens outside stability limits. 
  • Negative results in the setting of a respiratory illness may be due to infection with pathogens that are not detected by this test, or lower respiratory tract infection that may not be detected by an NPS specimen. Positive results do not rule out coinfection with other organisms. The agent(s) detected by the BIOFIRE RP2.1 may not be the definite cause of disease. Additional laboratory testing (e.g. bacterial and viral culture, immunofluorescence, and radiography) may be necessary when evaluating a patient with possible respiratory tract infection.

Reference Values

 

Analyte

Age

Sex

Reference Range

SARS-CoV-2 (COVID-19)

All

All

Not Detected

Influenza A, Influenza A subtypes H1,H3, and H1-2009

All

All

Not Detected

Influenza B

All

All

Not Detected

Respiratory Syncytial Virus (RSV)

All

All

Not Detected

Parainfluenza viruses type 1-4

All

All

Not Detected

Adenovirus

All

All

Not Detected

Metapneumovirus

All

All

Not Detected

Coronavirus HKU1

All

All

Not Detected

Coronavirus NL63

All

All

Not Detected

Coronavirus 229E

All

All

Not Detected

Coronavirus OC43

All

All

Not Detected

Enterovirus/Rhinovirus

All

All

Not Detected

Mycoplasma pneumoniae

All

All

Not Detected

Chlamydophila pneumoniae

All

All

Not Detected

Bordetella pertussis

All

All

Not Detected

Bordetella parapertussis

All

All

Not Detected

Critical Values

Analyte

Result
Bordetella pertussis

Detected

Turnaround Time

Turnaround times are relative to the time the specimen is received in the test laboratory. 

STAT Turnaround Time: not available

Routine Turnaround Time: 3 hours

Testing Schedule: Monday - Sunday

Clinical Indications

  • Simultaneous qualitative detection and identification of multiple respiratory viral and bacterial nucleic acids in nasopharyngeal swabs (NPS) obtained from individuals suspected of respiratory tract infections, including COVID-19.

CPT Codes

300002020 - 0202U, 30687633, 30587798 × 4

LOINC Codes

30687176, 30587205

Methodology

BioFire® automated Multiplex Polymerase Chain Reaction (PCR)

Last Review Date

09/27/2025