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HelpTest Code BCR ABL-1 Kinase Mutation Analysis Sequencing (Blood, Send out)
Specimen and Container/Tube
Blood
Rejection Criteria
Inappropriate anticoagulant and >48 hours, Poor RNA yield
Turnaround Time
Not Available
Test Includes
Detects greater than 85% of the reported ABL mutations (amino
acid residues 235 through 368)that lead to imatinib resistance.
Specimen Type
Blood
Preferred Volume
5 mL
Test Usage
Indicated in chronic myelogenous leukemia and Philadelphia
chromosome positive acute lymphocytic leukemia that have become
resistant to the imatinib drug.
Fee Code
376555-31081170
Offsite Collection Instructions
Protect from high or low temperatures. Send within 24 hours.
UCMC Collection Instructions
Send within 24 hours
Test Limitations
Will not detect mutation outside of amino acid 235-368 and
tumor
burden of <1 cell in 1,000 normal cells
STAT Turnaround Time
Not Available
Container/Tube
Lavender top
Specimen Minimum Volume
5 mL
Special Instructions
Refrigerate and send within 48 hours
Day(s) Performed
NA
STAT DAY(S) AND TIME(S) PERFORMED
No
CPT
81170
Method Name
RNA based. Reverse Transcription (RT)-PCR
Reference Values
Interpretative report, quantitative result will be provided
if
PCR product is
present.
Reject Due To
Inappropriate anticoagulant and >48 hours, Poor RNA yield
Pediatric Volume
5 mL
Clinical Indications
Indicated in chronic myelogenous leukemia and Philadelphia
chromosome positive acute lymphocytic leukemia that have become
resistant to the imatinib drug.
Transport Instructions
Protect from high or low temperatures. Send within 24 hours.
Test Components
Detects greater than 85% of the reported ABL mutations (amino
acid residues 235 through 368)that lead to imatinib resistance.
Methodology
RNA based.
Reverse Transcription
(RT)-PCR