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The University of Chicago Medicine Laboratories

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Mayo Clinic Laboratories

Test Code TPPA Syphilis Antibody, Treponema pallidum Particle Agglutination, Serum

Testing Algorithm

For more information see Syphilis Serology Algorithm.

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Specimen Type

Serum


Ordering Guidance


This assay is recommended by the Centers for Disease Control and Prevention for sera testing positive by a screening treponemal assay and negative by rapid plasma reagin (RPR). The results of this assay assist in determining whether the results of a screening treponemal test are truly or falsely positive.

 

For prenatal syphilis screening, the syphilis IgG test (SYPH1 / Syphilis IgG with Reflex, Enzyme Immunoassay, Serum) is recommended. Testing for IgM-class antibodies to T pallidum should not be performed during routine pregnancy screening.



Specimen Required


Supplies: Sarstedt Aliquot Tube 5 mL (T914)

Collection Container/Tube:

Preferred: Serum gel

Acceptable: Red top

Submission Container/Tube: Plastic vial

Specimen Volume: 0.5 mL

Collection Instructions: Centrifuge and aliquot serum into a plastic vial.


Specimen Minimum Volume

0.3 mL

Specimen Stability Information

Specimen Type Temperature Time
Serum Refrigerated (preferred) 14 days
  Frozen  14 days

Reference Values

Negative

Day(s) Performed

Monday through Friday

CPT Code Information

86780

Special Instructions

Report Available

Same day/1 to 4 days

Reject Due To

Gross hemolysis Reject
Gross lipemia Reject

Method Name

Particle Agglutination

Forms

If not ordering electronically, complete, print, and send Infectious Disease Serology Test Request (T916) with the specimen.