Test Code Quest 92810 HPV mRNA E6/E7, Rectal with Reflex to Genotypes, 16, 18/45 (Quest)
Specimen and Container/Tube
Specimen Type:
Anal/rectal specimen collected using an anal/rectal brush and submitted in 3 mL Preservcyt® transport medium ThinPrep® vial
Liquid Cytology (PreservCyt®) Preservative (ThinPrep®) vial
Specimen Handling and Transport Instructions
Room Temperature (preferred)
Collection Instructions
To collect an anal-rectal sample, a wetted, non-lubricated Dacron/Polyester swab is used. The Dacron/Polyester swab is inserted about 3 cm (or until resistance is met) into the anal canal past the anal verge, into the rectal vault. This is done without visualization of the anal canal. Firm lateral pressure is applied to the swab handle as it is rotated and slowly moved in and out. Slowly withdraw swab from the anal canal. Swish the swab vigorously in PreservCyt® fluid in the ThinPrep® vial. Discard the swab. Cap and tighten the ThinPrep® vial.
Follow the same procedure if using a brush to collect an anal-rectal sample. Avoid using cotton swab on a wooden stick because the handle may break and splinter during collection. Swab that is grossly contaminated with feces should be discarded and the collection re
Volume
Testing Volume: 3.0 mL
Minimum Testing Volume: 1.5 mL
Specimen Stability
Room temperature: 30 days
Refrigerated: 30 days
Frozen: Not established
Clinical Indications
HPV mRNA E6/E7, Rectal with Reflex to Genotypes, 16, 18/45 - The HPV mRNA E6/E7 test is used in conjunction with cytology to evaluate anal dysplasia.
Methodology
Transcription-Mediated Amplification (TMA)
Turnaround Time
TAT: 4 days
Offset: 7 days
Synonyms/Keyword Search
LABRFQSTHPV
LOINC Codes
74763-4
Reference Values
Not Detected