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The University of Chicago Medicine Laboratories

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Specimen Collection and Handling

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Mayo Clinic Laboratories

Test Code PTHB Parathyroid Hormone

Specimen and Container/Tube

Specimen Type: Plasma

Preferred Specimen: Lavender Top

Other Acceptable specimens: None

Volume

Preferred Volume: 0.5 mL

Minimum Volume: 0.2 mL

Specimen Stability

Storage Temperature

Stability

Room Temperature

2 days

Refrigerated

3 days 

Frozen

6 months 

Collection Instructions

Collect specimen per standard laboratory collection procedures. 

 

Specimen Handling and Transport Instructions

UCMC Onsite Instructions: 

  • Collect specimen per standard collection procedure and send to laboratory immediately. 
  • Send whole blood to the laboratory for analysis. 

 

UCMC Offsite and UC MedLab Outreach Instructions: 

  • Samples should be centrifuged within 2 hours of collection.
  • Centrifuge specimens at >2500 x g for 10 minutes at room temperature.
  • Aliquot sample into plastic transport tube containing a minimum of 0.2 mL of specimen.
  • Samples must be sent room temperature at 20-25oC.

Rejection Criteria

  • Common specimen rejected situations include: incorrect specimen type, insufficient volume, missing or incomplete specimen identifiers, incorrect specimen transport, or specimens outside stability limits. 
  • Specimens that are clotted or grossly hemolyzed.
  • Specimens that were transported unspun/whole blood and draw time is greater than 6 hours

Reference Values

Test

Age

Sex

Reference Range

Parathryroid Hormone

All

All

15 - 75 pg/mL

Critical Values

Not applicable 

Turnaround Time

Turnaround times are relative to the time the specimen is received in the test laboratory. 

 

STAT Turnaround Time: Not available as STAT test

Routine Turnaround Time: up to 4 hours

Testing Schedule:  24/7

Clinical Indications

PTH can be useful in aiding the differential diagnosis of hypercalcemia and hypocalcemia.

Test Components

Parathyroid hormone

Methodology

Electrochemiluminescence immunoassay (ECLIA) (Roche Diagnostics)

CPT Codes

83970

LOINC Codes

TBD

Synonyms/Keyword Search

PTH, Parathyroid hormone intact, PTI

Additional Information

  • There is no high-dose hook effect at PTH concentrations up to 17,000 pg/mL.
  • This test may be affected by biotin interference. Patients on high dose biotin (5mg/day or more) should not have samples drawn until at least 24 hours after the last biotin administration.

 

Last Review Date

6/4/25