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HelpTest Code LPMGF Lymphocyte Proliferation to Mitogens, Blood
Performing Laboratory
Mayo Clinic Laboratories in Rochester
Specimen Type
WB Sodium HeparinShipping Instructions
Testing performed Monday through Friday. Specimens not received by 4 p.m. Central time on Friday may be canceled.
Specimens arriving on the weekend and observed holidays may be canceled.
Collect and package specimen as close to shipping time as possible. Ship specimen overnight in an Ambient Shipping Box-Critical Specimens Only (T668) following the instructions in the box. It is recommended that specimens arrive within 24 hours of collection.
Necessary Information
1. Date and time of collection are required.
2. The ordering healthcare professional's name and phone number are required.
Specimen Required
Supplies: Ambient Shipping Box-Critical Specimens Only (T668)
Container/Tube: Green top (sodium heparin)
Specimen Volume: 20 mL
See tables for information on recommended volume based on absolute lymphocyte count
Pediatric Volume:
<3 months: 1 mL
3-24 months: 3 mL
25 months-18 years: 5 mL
Collection Instructions: Send whole blood specimen in original tube. Do not aliquot.
Additional Information: For serial monitoring, it is recommended that specimen collection be performed at the same time of day.
Table. Blood Volume Recommendations Based on Absolute Lymphocyte Count (ALC)
|
Mitogen only |
|||
|
ALC x 10(9)/L |
Blood volume for minimum phytohemagglutinin (PHA) only |
Blood volume for minimum PHA and pokeweed mitogen (PWM) |
Blood volume for full assay |
|
<0.5 |
>6.5 mL |
>8.5 mL |
>22 mL |
|
0.5-1.0 |
6.5 mL |
8.5 mL |
22 mL |
|
1.1-1.5 |
3.0 mL |
4.0 mL |
10 mL |
|
1.6-2.0 |
2.0 mL |
2.5 mL |
7 mL |
|
2.1-3.0 |
1.5 mL |
2.0 mL |
6 mL |
|
3.1-4.0 |
1.0 mL |
1.5 mL |
4 mL |
|
4.1-5.0 |
0.8 mL |
1.0 mL |
3 mL |
|
>5.0 |
0.5 mL |
0.8 mL |
2 mL |
|
Mitogen and antigen |
||
|
ALC x 10(9)/L |
Blood volume for minimum of each assay |
Blood volume for full assay |
|
<0.5 |
>28 mL |
>60 mL |
|
0.5-1.0 |
28 mL |
60 mL |
|
1.1-1.5 |
12 mL |
30 mL |
|
1.6-2.0 |
8.5 mL |
20 mL |
|
2.1-3.0 |
6.5 mL |
15 mL |
|
3.1-4.0 |
4.5 mL |
10 mL |
|
4.1-5.0 |
3.5 mL |
8 mL |
|
>5.0 |
2.5 mL |
6 mL |
Specimen Minimum Volume
See Specimen Required
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| WB Sodium Heparin | Ambient | 48 hours | GREEN TOP/HEP |
Reference Values
Viability of lymphocytes at day 0: ≥75.0%
Maximum proliferation of phytohemagglutinin as % CD45: ≥49.9%
Maximum proliferation of phytohemagglutinin as % CD3: ≥58.5%
Maximum proliferation of pokeweed mitogen as % CD45: ≥4.5%
Maximum proliferation of pokeweed mitogen as % CD3: ≥3.5%
Maximum proliferation of pokeweed mitogen as % CD19: ≥3.9%
Day(s) Performed
Monday through Friday
CPT Code Information
86353
86353 (if appropriate)
Report Available
8 to 11 daysReject Due To
| Gross hemolysis | Reject |
| Gross lipemia | Reject |
Method Name
Flow Cytometry
Reflex Tests
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| MGSTM | Additional Flow Stimulant, LPMGF | No, (Bill Only) | No |
Testing Algorithm
To ensure the most reliable results, if insufficient peripheral blood mononuclear cells are isolated from the patient's sample due to low white blood cell counts or specimen volume received, selected dilutions or stimulants may not be tested at the discretion of the laboratory.
Testing with one stimulant will always be performed. When adequate specimen is available for both stimulants to be tested, the second stimulant will be evaluated at an additional charge.