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Test Code HIVSD HIV Antibody Supplemental/Differentiation

Important Note

Please see the HIV testing algorithm to the left, under 'Resources'.

Infectious

Specimen and Container/Tube

Specimen Type: Whole blood

Preferred Specimen: Lavender-top tube

Other Acceptable specimens: Gold-top SST

Volume

Preferred Volume: 10 mL

Minimum Volume: 8 mL

 

Specimen Stability

Storage Temperature

Stability

Room temperature

(20 - 25°C)

 2 days

Refrigerated

(2 - 8°C)

 7 days

Frozen

(-20°C)

not acceptable

Collection Instructions

Specimen Collection: Collect blood from venipuncture by aseptic technique.


Specimen Transport: Immediate

Specimen Handling and Transport Instructions

UCMC Onsite Instructions: Collect specimen per standard collection procedure and send to laboratory immediately. 

 

UCMC Offsite and UC MedLab Outreach Instructions: Collect specimen per standard collection procedure and send to laboratory immediately.

Rejection Criteria

  • Common specimen rejected situations include: incorrect specimen type, insufficient volume, incorrect collection container, missing or incomplete specimen identifiers, incorrect specimen transport, or specimens outside stability limits. 
  • Grossly hemolyzed, lipemic, or icteric specimens will be rejected.

Reference Values

 

Test

Age

Sex

Reference Range

HIV 1

All

All

 Negative
HIV 2 All All Negative

Turnaround Time

Turnaround times are relative to the time the specimen is received in the test laboratory. 

STAT Turnaround Time: not available

Routine Turnaround Time: 2 days

Testing Schedule: Monday - Sunday

Clinical Indications

  • Aid in the confirmation and differentiation of individual antibodies to Human Immunodeficiency Virus Types 1 and 2 (HIV-1 and HIV-2) in serum, or plasma samples.
  • Aid as an additional, more specific test to confirm the presence of antibodies to HIV-1 and HIV-2 for specimens found to be repeatedly reactive by diagnostic screening procedures
  • This assay may also be used to confirm the presence of antibodies to HIV-1 and/or HIV-2 in pediatric patients (i.e., children as young as 2 years of age).

Methodology

Immunochromatographic assay

CPT Codes

86701, 86702

LOINC Codes

30086701, 30086702

Synonyms/Keyword Search

LABISHIVSD, HIVD

Last Review Date

06/12/2026