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HelpTest Code GPT Alanine Aminotransferase (ALT/GPT)
Specimen and Container/Tube
Specimen Type: Plasma
Preferred Specimen: Li-Heparin Mint Green Top
Other Acceptable specimens: Serum/Red Top, Serum/Gold Top
Collection Instructions
Collect specimen per standard laboratory collection procedures.
Specimen Handling and Transport Instructions
UCMC Onsite Instructions:
Collect specimen per standard collection procedure and send to laboratory immediately.
UCMC Offsite and UC MedLab Outreach Instructions:
- Samples should be centrifuged within 2 hours of collection.
- Centrifuge specimens at >2500 x g for 10 minutes at room temperature.
- Aliquot sample into plastic transport tube containing a minimum of 0.2 mL of specimen.
- Samples must be sent room temperature at 15-25oC.
Reference Values
|
Test |
Age |
Sex |
Reference Range |
|
ALT (GPT) |
All |
All |
8-35 U/L |
Note: Reference range based on in-house studies, recommendations from Tietz Textbook of Clinical Chemistry, and Roche product literature.
Volume
Preferred Volume: 0.5 mL
Minimum Volume: 0.2 mL
Rejection Criteria
- Common specimen rejected situations include: incorrect specimen type, insufficient volume, missing or incomplete specimen identifiers, incorrect specimen transport, or specimens outside stability limits.
- Specimens that are clotted or grossly hemolyzed.
- Specimens that were transported unspun/whole blood and draw time is greater than 6 hours.
Critical Values
Not applicable
CPT Codes
84460
Specimen Stability
|
Storage Temperature |
Stability |
|
Room temperature |
3 days |
|
Refrigerated |
7 days |
|
Frozen |
7 days |
Test Components
Alanine Aminotransferase
Clinical Indications
Diagnosis and monitoring of liver disease associated with hepatic necrosis
Methodology
Photometric Rate, L-Alanine with Pyridoxal-5-Phosphate
Additional Information
Results should always be assessed in conjunction with patient's medical history, clinical examination and other
findings.
- Results should always be assessed in conjunction with patient's medical history, clinical examination and other findings.
- When blood is drawn and permitted to clot and to stand uncentrifuged at room temperature, the average decrease in serum glucose is approximately 7% in one hour (0.28 to 0.56 mmol/L or 5 to 10 mg/dL). This decrease is the result of glycolysis. Glycolysis can be inhibited by collecting the specimen in fluoride tubes.
- Patients can be fasting or non-fasting.
- Also included in Hepatic Function Panel LFT, Pediatric Hepatic Function Panel PDLPNL, Comprehensive Metabolic Panel CMP, ER Panel II STLP, Pediatric ER Panel II PDSTLP, CMP for chronic lymphocytic leukemia (CPNL FOR CLL PATIENTS ONLY) SSTCP.
Turnaround Time
Turnaround times are relative to the time the specimen is received in the test laboratory.
STAT Turnaround Time: 1 hour
Routine Turnaround Time: 4 hours
Testing Schedule: 24/7
Last Review Date
06/12/2024
LOINC Code
1743-4
Synonyms/Keyword Search
ALT, GPT