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The University of Chicago Medicine Laboratories

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Specimen Collection and Handling

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Mayo Clinic Laboratories

Test Code DAT Antiglobulin Test, Direct

Important Note

Can be an add-on if blood bank already has a pink top tube sample.

Specimen and Container/Tube

Pink top, EDTA

NOTE: All blood bank samples must have HAND WRITTEN collection information on the label to be accepted (date MM/DD/YY or MM/DD/YYYY, time, and initials). See example below:

 

Rejection Criteria

This sample will be rejected if not labeled properly:

  • Must be labeled with patient LAST name, FIRST name, and MRN.
  • All blood bank samples must have HAND WRITTEN collection information on the label to be accepted (date MM/DD/YY or MM/DD/YYYY, time, and initials).
  • All information must be legible.

Preferred Volume

6 mL

Pediatric Volume

2 mL

Clinical Indications

  • The DAT is a simple test to determine if red cells have been coated in vivo with immunoglobulin (Ig), complement, or both.
  • DAT is used in the investigation of:
    • hemolytic transfusion reactions
    • hemolytic disease of the fetus and newborn (HDFN)
    • autoimmune hemolytic anemia (AIHA)
    • drug-induced immune hemolysis

 

 

Test Components

  • Direct Antiglobulin Test (DAT), IgG
  • Complement C3

Last reviewed

Last reviewed 08/07/2024 RM