Test Code CALR CALR Mutation Analysis, Myeloproliferative Neoplasm (MPN), Varies
Special Instructions
Specimen Type
VariesOrdering Guidance
Shipping Instructions
Specimen must arrive within 7 days of collection.
Necessary Information
Specimen source is required
Specimen Required
Submit only 1 of the following specimens:
Specimen Type: Whole blood
Container/Tube: Lavender top (EDTA) or yellow top (ACD)
Specimen Volume: 3 mL
Collection Instructions:
1. Invert several times to mix blood.
2. Send whole blood specimen in original tube. Do not aliquot.
3. Label specimen as whole blood.
Specimen Stability Information: Ambient (preferred)/Refrigerate
Additional Information: To ensure minimum volume and concentration of DNA is met, the preferred volume of blood must be submitted. Testing may be canceled if DNA requirements are inadequate.
Specimen Type: Bone marrow
Container/Tube: Lavender top (EDTA) or yellow top (ACD
Specimen Volume: 2 mL
Collection Instructions:
1. Invert several times to mix bone marrow.
2. Send bone marrow specimen in original tube. Do not aliquot.
3. Label specimen as bone marrow.
Specimen Stability Information: Ambient (preferred)/Refrigerate
Specimen Type: Extracted DNA from blood or bone marrow
Container/Tube: 1.5- to 2-mL tube
Specimen Volume: Entire specimen
Collection Instructions:
1. DNA must be extracted from blood or bone marrow within 7 days of collection.
2. Label specimen as extracted DNA and source of specimen.
3. Provide volume and concentration of the DNA.
Specimen Stability Information: Frozen (preferred) 1 year/Refrigerate/Ambient
Additional Information: DNA must be extracted in a CLIA-certified laboratory or equivalent and must be extracted from a specimen type listed as acceptable for this test (including applicable anticoagulants). We cannot guarantee that all extraction methods are compatible with this test. If testing fails, one repeat will be attempted, and if unsuccessful, the test will be reported as failed and a charge will be applied.
Specimen Minimum Volume
Whole blood/bone marrow: 1 mL; Extracted DNA: 50 mcl at 20 ng/mcL concentration
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Varies | Varies | 7 days |
Reject Due To
| Gross hemolysis | Reject |
| Paraffin-embedded bone marrow aspirate clot | Reject |
| Bone marrow biopsies, slides, or paraffin shavings | Reject |
| Moderately to severely clotted | Reject |
Reference Values
An interpretive report will be provided
Day(s) Performed
Monday through Friday
Report Available
3 to 5 daysPerforming Laboratory
Mayo Clinic Laboratories in Rochester
CPT Code Information
81219-CALR (calreticulin) (eg, myeloproliferative disorders), gene analysis, common variants in exon 9
Testing Algorithm
For information see:
-Myeloproliferative Neoplasm: A Diagnostic Approach to Peripheral Blood Evaluation
-Myeloproliferative Neoplasm: A Diagnostic Approach to Bone Marrow Evaluation
Method Name
Polymerase Chain Reaction (PCR) and Fragment Analysis
Forms
1. Hematopathology Patient Information (T676)
2. If not ordering electronically, complete, print, and send a Hematopathology/Cytogenetics Test Request (T726) with the specimen.