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Test Code AMITR Amikacin, Trough

Specimen and Container/Tube

Specimen Type: Serum

Preferred Specimen: Red Top

Other Acceptable specimens: Serum/Gold Top

Specimen Stability

Storage Temperature

Stability

Room temperature

8 hours

Refrigerated

2 days

Frozen

4 weeks

Collection Instructions

Collect specimen per standard laboratory collection procedures. 

 

Specimen Handling and Transport Instructions

UCMC Onsite Instructions: 

Collect specimen per standard collection procedure and send to laboratory immediately. 

 

UCMC Offsite and UC MedLab Outreach Instructions: 

  • Samples should be centrifuged within 2 hours of collection.
  • Centrifuge specimens at >2500 x g for 10 minutes at room temperature.
  • Aliquot sample into plastic transport tube containing a minimum of 0.2 mL of specimen.
  • Samples must be sent refrigerated at 2-8oC.

Rejection Criteria

  • Common specimen rejected situations include: incorrect specimen type, insufficient volume, missing or incomplete specimen identifiers, incorrect specimen transport, or specimens outside stability limits. 
  • Specimens that are clotted or grossly hemolyzed.
  • Specimens that were transported unspun/whole blood and draw time is greater than 6 hours.

Reference Values

Test

Age

Sex

Reference Range

Amikacin

All

All

<4 μg/mL

Critical Values

Analyte

Unit

Low

High

Age

Amikacin

μg/mL

N/A

>10.0

All

Turnaround Time

Turnaround times are relative to the time the specimen is received in the test laboratory. 

 

STAT Turnaround Time: 1 hour

Routine Turnaround Time: 4 hours

Testing Schedule: 24/7

Clinical Indications

Determination of serum or plasma drug levels is required to achieve optimum therapeutic efficacy and minimize toxicity.

Test Components

Amikacin
 

CPT Codes

80150

LOINC Codes

3321-7

Methodology

Kinetic Interaction of Microparticles in solution (KIMS) (Roche Diagnostics)

Synonyms/Keyword Search

N/A

Additional Information

  • Results should always be assessed in conjunction with patient's medical history, clinical examination and other findings.
  • Determination of serum or plasma drug levels is required to achieve optimum therapeutic efficacy and minimize toxicity.
  • Serum tubes containing separating gel may be used if received in Labortory Services within 2 hours after collection.
  • Collection time varies dependent upon desired measurement of peak or trough values.

Last Review Date

8/14/2024

Volume

Preferred Volume: 0.5 mL

Minimum Volume: 0.2 mL

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