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Mayo Clinic Laboratories

Test Code UIEX Urine Protein Electrophoresis and Immunifixation, Timed

Important Note

If ordering random test, used test RUIEX. 

Specimen and Container/Tube

Specimen Type: Urine, 24-hour Times Collection 

Preferred Specimen: 24 Hour Sterile Plastic Container

Other Acceptable specimens: Sterile Clean Glass container

Specimen Stability

Storage Temperature

Stability

Room temperature

2 hours

Refrigerated

7 days

Frozen

1 month

Collection Instructions

Collect specimen per standard laboratory collection procedures. 

 

Specimen Handling and Transport Instructions

UCMC Onsite Instructions: 

  • Collect specimen per standard collection procedure and send to laboratory immediately. 
  • No preservatives or additives.

 

UCMC Offsite and UC MedLab Outreach Instructions: 

  • Collect specimen per standard collection procedure and send to laboratory immediately. 
  • No preservatives or additives. 
  • Aliquot urine into plastic tube containing a minimum of 10.0 mL of specimen.
  • Urine samples must then be sent refrigerated at 2-8oC.

Rejection Criteria

  • Common specimen rejected situations include: incorrect specimen type, insufficient volume, missing or incomplete specimen identifiers, incorrect specimen transport, or specimens outside stability limits. 
  • Do not use boric acid, acetic acid or hydrochloric acid as a preservative.

Reference Values

 

Test

Age

Sex

Reference Range

All test components 

All

All

Not applicable

 

Critical Values

Not applicable

Turnaround Time

Turnaround times are relative to the time the specimen is received in the test laboratory. 

 

STAT Turnaround Time: Not available as STAT test

Routine Turnaround Time: 48 hours

Testing Schedule: Monday - Friday

Clinical Indications

Urine protein electrophoresis may help identify monoclonal proteins which are excreted into the urine and may elude detection by serum protein electrophoresis, especially the Bence-Jones Proteins (monoclonal free kappa or lambda light chains) which are characteristic of light chain disease.

Test Components

Urine collection duration, Urine total volume, Urine protein random, Urine protein timed,  Urine albumin percent, Protein Electrophoresis, Immunofixation Electrophoresis with urine concentration for IgG, IgA, IgM, Kappa and Lambda.Pathologist interpretation 

 

Methodology

Endpoint Colorimetric Assay Spectrophotometric (Roche Diagnostics)

Sebia Hydragel (HYDRASYS LC and PHORESIS) Electrophoresis System

Sebia Capillarys 3 Tera 

CPT Codes

84166, 86335, 84156, 84166

LOINC Codes

TBD

Additional Information

  • Fresh 24 hour urine or first morning catch is preferred to avoid possible interferences such as contamination with bacteria, increase in pH due to breakdown of urea to ammonia or degradation of proteins by proteases.

Last Review Date

4/18/2025

Volume

Preferred Volume: 10.0 mL

Minimum Volume: 5.0 mL