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Test Code TTEIA Total Testosterone Immunoassay

Specimen and Container/Tube

Specimen Type: Serum

Preferred Specimen: Red Top

Other Acceptable specimens: Serum/Gold Top, Li-Heparin Mint Green Top

Volume

Preferred Volume: 4.0 mL

Minimum Volume: 0.2 mL

Specimen Stability

Storage Temperature

Stability

Room temperature

7 days

Refrigerated

7 days

Frozen

3 months

Collection Instructions

Collect specimen per standard laboratory collection procedures.

 

Specimen Handling and Transport Instructions

UCMC Onsite Instructions: 

Collect specimen per standard collection procedure and send to laboratory immediately. 

 

UCMC Offsite and UC MedLab Outreach Instructions: 

  • Samples should be centrifuged within 2 hours of collection.
  • Centrifuge specimens at >2500 x g for 10 minutes at room temperature.
  • Aliquot sample into plastic transport tube containing a minimum of 0.2 mL of specimen.
  • Samples must be sent room temperature at 15-25oC.

Rejection Criteria

  • Common specimen rejected situations include: incorrect specimen type, insufficient volume, missing or incomplete specimen identifiers, incorrect specimen transport, or specimens outside stability limits. 
  • Specimens that are clotted or grossly hemolyzed.
  • Specimens that were transported unspun/whole blood and draw time is greater than 6 hours.

Reference Values

Test

Age

Sex

Reference Range

Total Testosterone

0 - 5 months

Male

75 - 400 ng/dL

6 months - 9 years

<20 ng/dL

10 - 11 years

<130 ng/dL

12 - 13 years

<800 ng/dL

14 years

<1,200 ng/dL

15 - 16 years

100 - 1,200 ng/dL

17 - 18 years

300 - 1,200 ng/dL

19 - 55 years

240 - 950 ng/dL

>55 years

180 - 800 ng/dL

 

Test

Age

Sex

Reference Range

Total Testosterone

0 - 5 months

Female

20 - 80 ng/dL

6 months - 9 years

<20 ng/dL

10 - 11 years

<44 ng/dL

12 - 16 years

15 - 60 ng/dL

17 - 40 years

20 - 60 ng/dL

41 - 60 years

<60 ng/dL

Post-menopausal (>60 yrs)

<70 ng/dL

Critical Values

Not applicable

Test Components

Testosterone, Total

Methodology

Electrochemiluminescence immunoassay (ECLIA) (Roche Diagnostics)

CPT Codes

84403

LOINC Codes

2986-8

Additional Information

  • Results should always be assessed in conjunction with patient's medical history, clinical examination and other findings. 

Turnaround Time

Turnaround times are relative to the time the specimen is received in the test laboratory. 

 

STAT Turnaround Time: Not Available as STAT

Routine Turnaround Time: 4 hours

Testing Schedule: 24/7

Synonyms/Keyword Search

N/A

Last Review Date

8/13/2025

Clinical Indications

Monitor total testosterone serum levels in males and females.