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Test Code TTEIA Total Testosterone Immunoassay

Specimen and Container/Tube

Specimen Type: Serum

Preferred Specimen: Red Top

Other Acceptable specimens: Serum/Gold Top, Li-Heparin Mint Green Top

Specimen Stability

Storage Temperature

Stability

Room temperature

7 days

Refrigerated

7 days

Frozen

3 months

Collection Instructions

Collect specimen per standard laboratory collection procedures.

 

Specimen Handling and Transport Instructions

UCMC Onsite Instructions: 

Collect specimen per standard collection procedure and send to laboratory immediately. 

 

UCMC Offsite and UC MedLab Outreach Instructions: 

  • Samples should be centrifuged within 2 hours of collection.
  • Centrifuge specimens at >2500 x g for 10 minutes at room temperature.
  • Aliquot sample into plastic transport tube containing a minimum of 0.2 mL of specimen.
  • Samples must be sent room temperature at 15-25oC.

Rejection Criteria

  • Common specimen rejected situations include: incorrect specimen type, insufficient volume, missing or incomplete specimen identifiers, incorrect specimen transport, or specimens outside stability limits. 
  • Specimens that are clotted or grossly hemolyzed.
  • Specimens that were transported unspun/whole blood and draw time is greater than 6 hours.

Reference Values

Test

Age

Sex

Reference Range

Total Testosterone

0 - 5 months

Male

75 - 400 ng/dL

6 months - 9 years

<20 ng/dL

10 - 11 years

<130 ng/dL

12 - 13 years

<800 ng/dL

14 years

<1,200 ng/mL

15 - 16 years

100 - 1,200 ng/dL

17 - 18 years

300 - 1,200 ng/dL

19 - 55 years

240 - 950 ng/dL

56 - 75 years

180 - 800 ng/dL

 

Test

Age

Sex

Reference Range

Total Testosterone

0 - 5 months

Female

20 - 80 ng/dL

6 months - 9 years

<20 ng/dL

10 - 11 years

<44 ng/dL

12 - 16 years

15 - 60 ng/dL

17 - 40 years

20 - 60 ng/mL

41 - 60 years

<60 ng/dL

Post-menopausal (>60 yrs)

5 - 70 years

Critical Values

Not applicable

Turnaround Time

Turnaround times are relative to the time the specimen is received in the test laboratory. 

 

STAT Turnaround Time: Not Available as STAT

Routine Turnaround Time: 4 hours

Testing Schedule: 24/7

Clinical Indications

Monitor total testosterone serum levels in males and females.

Test Components

Testosterone, Total

CPT Codes

84403

LOINC Codes

2986-8

Methodology

Electrochemiluminescence immunoassay (ECLIA) (Roche Diagnostics)

Synonyms/Keyword Search

N/A

Additional Information

  • Results should always be assessed in conjunction with patient's medical history, clinical examination and other findings. 

Last Review Date

8/13/2025

Volume

Preferred Volume: 4.0 mL

Minimum Volume: 0.2 mL