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The University of Chicago Medicine Laboratories

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Specimen Collection and Handling

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Mayo Clinic Laboratories

Test Code STEM CD34 Stem Cell Count

Important Note

Monday - Friday 8:00 am - 5:00 pm and Saturday 8:00 am - 3:00 pm, if later specimens are expected the laboratory must be notified

Specimen and Container/Tube

Specimen Type: Whole blood

Preferred Specimen: lavender top

Other Acceptable specimens: Stem Cell Transplant Products,
 Bone Marrow Product, ACD and Heparinized samples

Volume

Preferred Volume: 4.0 mL

Minimum Volume: 1.0 mL

Specimen Stability

Storage Temperature

Stability

Room temperature

(20 - 25°C)

 24 hours 

Refrigerated

(2 - 10°C)

 not acceptable

Frozen

(-20°C)

not acceptable

Collection Instructions

Collect specimen per standard laboratory collection procedures. 

Specimen Handling and Transport Instructions

UCMC Onsite Instructions: Collect specimen per standard collection procedure and send to laboratory immediately.

UCMC Offsite and UC MedLab Outreach Instructions: Collect specimen per standard collection procedure and send to laboratory immediately. Specimens must be received within 24 hours. If transport is delayed, retain specimen at room temperature. 

Rejection Criteria

  • Common specimen rejected situations include: incorrect specimen type, insufficient volume, missing or incomplete specimen identifiers, incorrect specimen transport, or specimens outside stability limits. 
  • Frozen or heated specimens will be rejected
  • Clotted or quantity not sufficient specimens are not suitable for testing and will be rejected 
  • Stem count analysis is not performed on specimens with WBC counts <1.0 K/uL, unless the specimen is a CD34 selection or if preapproved by the Medical Director of Flow Cytometry.

 

Reference Values

not applicable 

Critical Values

not applicable

Clinical Indications

  • Stem cell transplant

Test Components

WBC, percent CD34+ cells, absolute CD34+ cells, percent CD34+ cells 7AAD-

Methodology

Flow cytometry

CPT Codes

86367

LOINC Codes

WBC, CD34P 33719-6, CD34A 33719-6, 7AAD 33719-6

Turnaround Time

Turnaround times are relative to the time the specimen is received in the test laboratory. 

 

STAT Turnaround Time: 90 minutes 

 

Routine Turnaround Time: 240 minutes

 

Testing Schedule: Monday - Friday 8:00 am - 5:00 pm and Saturday 8:00 am - 3:00 pm, if later specimens are expected the laboratory must be notified

Synonyms/Search Keywords

Stem cell count

Last Reviewed Date

8/12/2024