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Test Code STEM CD34 Stem Cell Count

Important Note

Monday - Friday 8:00 am - 5:00 pm and Saturday 8:00 am - 3:00 pm, if later specimens are expected the laboratory must be notified

Specimen and Container/Tube

Specimen Type: Whole blood

Preferred Specimen: lavender top

Other Acceptable specimens: Stem Cell Transplant Products,
 Bone Marrow Product, ACD and Heparinized samples

Specimen Handling and Transport Instructions

UCMC Onsite Instructions: Collect specimen per standard collection procedure and send to laboratory immediately.

UCMC Offsite and UC MedLab Outreach Instructions: Collect specimen per standard collection procedure and send to laboratory immediately. Specimens must be received within 24 hours. If transport is delayed, retain specimen at room temperature. 

Rejection Criteria

  • Common specimen rejected situations include: incorrect specimen type, insufficient volume, missing or incomplete specimen identifiers, incorrect specimen transport, or specimens outside stability limits. 
  • Frozen or heated specimens will be rejected
  • Clotted or quantity not sufficient specimens are not suitable for testing and will be rejected 
  • Stem count analysis is not performed on specimens with WBC counts <1.0 K/uL, unless the specimen is a CD34 selection or if preapproved by the Medical Director of Flow Cytometry.

 

Reference Values

not applicable 

Critical Values

not applicable

Turnaround Time

Turnaround times are relative to the time the specimen is received in the test laboratory. 

 

STAT Turnaround Time: 90 minutes 

 

Routine Turnaround Time: 240 minutes

 

Testing Schedule: Monday - Friday 8:00 am - 5:00 pm and Saturday 8:00 am - 3:00 pm, if later specimens are expected the laboratory must be notified

CPT Codes

86367

LOINC Codes

WBC, CD34P 33719-6, CD34A 33719-6, 7AAD 33719-6

Volume

Preferred Volume: 4.0 mL

Minimum Volume: 1.0 mL

Specimen Stability

Storage Temperature

Stability

Room temperature

(20 - 25°C)

24 hours 

Refrigerated

(2 - 10°C)

not acceptable

Frozen

(-20°C)

not acceptable

Collection Instructions

Collect specimen per standard laboratory collection procedures. 

Test Components

WBC, percent CD34+ cells, absolute CD34+ cells, percent CD34+ cells 7AAD-

Clinical Indications

  • Stem cell transplant

Methodology

Flow cytometry

Synonyms/Search Keywords

Stem cell count

Last Reviewed Date

8/12/2024