Test Code STEM CD34 Stem Cell Count
Specimen and Container/Tube
Specimen Type: Whole blood
Preferred Specimen: lavender top
Other Acceptable specimens: Stem Cell Transplant Products,
Bone Marrow Product, ACD and Heparinized samples
Volume
Preferred Volume: 4.0 mL
Minimum Volume: 1.0 mL
Specimen Stability
Storage Temperature |
Stability |
Room temperature (20 - 25°C) |
24 hours |
Refrigerated (2 - 10°C) |
not acceptable |
Frozen (-20°C) |
not acceptable |
Collection Instructions
Collect specimen per standard laboratory collection procedures.
Specimen Handling and Transport Instructions
UCMC Onsite Instructions: Collect specimen per standard collection procedure and send to laboratory immediately.
UCMC Offsite and UC MedLab Outreach Instructions: Collect specimen per standard collection procedure and send to laboratory immediately. Specimens must be received within 24 hours. If transport is delayed, retain specimen at room temperature.
Rejection Criteria
- Common specimen rejected situations include: incorrect specimen type, insufficient volume, missing or incomplete specimen identifiers, incorrect specimen transport, or specimens outside stability limits.
- Frozen or heated specimens will be rejected
- Clotted or quantity not sufficient specimens are not suitable for testing and will be rejected
- Stem count analysis is not performed on specimens with WBC counts <1.0 K/uL, unless the specimen is a CD34 selection or if preapproved by the Medical Director of Flow Cytometry.
Reference Values
not applicable
Critical Values
not applicable
Clinical Indications
- Stem cell transplant
Test Components
WBC, percent CD34+ cells, absolute CD34+ cells, percent CD34+ cells 7AAD-
Methodology
Flow cytometry
CPT Codes
86367
LOINC Codes
WBC, CD34P 33719-6, CD34A 33719-6, 7AAD 33719-6
Turnaround Time
Turnaround times are relative to the time the specimen is received in the test laboratory.
STAT Turnaround Time: 90 minutes
Routine Turnaround Time: 240 minutes
Testing Schedule: Monday - Friday 8:00 am - 5:00 pm and Saturday 8:00 am - 3:00 pm, if later specimens are expected the laboratory must be notified
Synonyms/Search Keywords
Stem cell count
Last Reviewed Date
8/12/2024