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The University of Chicago Medicine Laboratories

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Specimen Collection and Handling

Transfusion

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Mayo Clinic Laboratories

Test Code RTPVP Blood Gas, Venous ECMO Pump (Pediatric)

Specimen and Container/Tube

Specimen Type: Whole blood 

Preferred Specimen: Syringe

Other Acceptable Specimens: None

Volume

Preferred Volume: 0.5 mL

Minimum Volume: 0.2 mL

Specimen Stability

Storage Temperature

Stability

Room temperature

30 minutes

Refrigerated

not acceptable

Frozen

not acceptable

Collection Instructions

  • Collect specimen per standard laboratory collection procedures. 
  • Remove all air from syringe. 
  • Remove needle. 
  • Cap tightly and mix well. 

Specimen Handling and Transport Instructions

UCMC Onsite Instructions: 

  • Collect specimen per standard collection procedure and send to laboratory immediately. 
  • Testing must be performed within 30 minutes of collection 

UCMC Offsite and UC MedLab Outreach Instructions: 

  • Specimens are not acceptable from offsite locations for blood gas testing 

Rejection Criteria

  • Common specimen rejected situations include: incorrect specimen type, insufficient volume, missing or incomplete specimen identifiers, incorrect specimen transport, or specimens outside stability limits. 
  • Specimens with needles attached will be rejected. 
  • Specimens with air bubbles. 

Reference Values

Not applicable 

Critical Values

Not applicable 

Turnaround Time

Turnaround times are relative to the time the specimen is received in the test laboratory. 

 

STAT Turnaround Time: 1 hour

Routine Turnaround Time: 4 hours

Testing Schedule: 24/7

Test Components

pH, PCO2, PO2, HCO3-, Base Excess, SO2, SVO2, Hemoglobin, Oxyhemoglobin, Deoxyhemoglobin, Carboxyhemoglobin, Methemoglobin

Methodology

Potentiometry: pH, pCO2

Amperometry: pO2

Derived: HCO3-, base excess

Optical (CO-oximetry): SO2, hemoglobin, oxyhemoglobin, deoxyhemoglobin, carboxyhemoglobin, methemoglobin

CPT Codes

82805

LOINC Codes

TBD

Additional Information

  • For SO2: Measured oxygen saturation of functional hemoglobin according to the following formula. OxyHb/(OxyHb + DeoxyHb).
  • For OxyHb: Measured oxygen saturation of total hemoglobin according to the following formula. OxyHb/(OxyHb + DeoxyHb + COHb + MetHb).

Last Review Date

4/29/2025