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The University of Chicago Medicine Laboratories

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Specimen Collection and Handling

Transfusion

Forms

 

 

 

 

Mayo Clinic Laboratories

Test Code RTBGPP Blood Gas, Arterial ECMO Pump 

Specimen and Container/Tube

Specimen Type: Whole blood 

Preferred Specimen: Syringe arterial 

Other Acceptable Specimens: None

Specimen Handling and Transport Instructions

UCMC Onsite Instructions: 

  • Collect specimen per standard collection procedure and send to laboratory immediately. 
  • Testing must be performed within 30 minutes of collection 

UCMC Offsite and UC MedLab Outreach Instructions: 

  • Specimens are not acceptable from offsite locations for blood gas testing 

Rejection Criteria

  • Common specimen rejected situations include: incorrect specimen type, insufficient volume, missing or incomplete specimen identifiers, incorrect specimen transport, or specimens outside stability limits. 
  • Specimens with needles attached will be rejected. 
  • Specimens with air bubbles. 

Reference Values

Analyte

Age

Sex

Normal Range

SO2, arterial ECMO pump

All

All

94 -98%

Hemoglobin, arterial ECMO pump

All

All

≥6.0 g/dL

Methemoglobin, arterial ECMO pump

All

All

≤2.0%

Critical Values

Analyte

Age

Low

High

Hemoglobin, arterial

All

<6.0 g/dL

NA

Methemoglobin

All

NA

>10.0%

CPT Codes

82805

LOINC Codes

TBD

Volume

Preferred Volume: 0.5 mL

Minimum Volume: 0.2 mL

Specimen Stability

Storage Temperature

Stability

Room temperature

30 minutes

Refrigerated

not acceptable

Frozen

not acceptable

Collection Instructions

  • Collect specimen per standard laboratory collection procedures. 
  • Remove all air from syringe. 
  • Remove needle. 
  • Cap tightly and mix well. 

Test Components

pH, PCO2, PO2, HCO3-, Base Excess, SO2, SAO2, Hemoglobin, Oxyhemoglobin, Deoxyhemoglobin, Carboxyhemoglobin, Methemoglobin

Methodology

Potentiometry: pH, pCO2

Amperometry: pO2

Derived: HCO3-, base excess

Optical (CO-oximetry): SO2, hemoglobin, oxyhemoglobin, deoxyhemoglobin, carboxyhemoglobin, methemoglobin

Additional Information

  • For SO2: Measured oxygen saturation of functional hemoglobin according to the following formula. OxyHb/(OxyHb + DeoxyHb).
  • For OxyHb: Measured oxygen saturation of total hemoglobin according to the following formula. OxyHb/(OxyHb + DeoxyHb + COHb + MetHb).

Turnaround Time

Turnaround times are relative to the time the specimen is received in the test laboratory. 

 

STAT Turnaround Time: 1 hour

Routine Turnaround Time: 4 hours

Testing Schedule: 24/7

Last Review Date

4/29/2025