Sign in →

Test Code PRA Renin Activity, Plasma

Important Note

Use Sunquest code RENINS to order

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Specimen Type

Plasma EDTA


Specimen Required


Patient Preparation: The plasma renin activity cannot be interpreted if the patient is being treated with spironolactone (Aldactone). Spironolactone should be discontinued for 4 to 6 weeks before testing.

Collection Container/Tube: Chilled, lavender top (EDTA)

Submission Container/Tube: Plastic vial

Specimen Volume: 2 mL

Collection Instructions:

1. Draw blood in a chilled syringe from a patient in a seated position; place specimen in a chilled, lavender-top (EDTA) tube; and mix.

2. Alternatively, draw blood directly in a chilled, lavender top (EDTA) tube.

3. Immediately place EDTA tube into an ice-water bath until thoroughly cooled.

4. Refrigerate specimen during centrifugation and immediately transfer plasma to plastic vial. (If a refrigerated centrifuge is unavailable, chill the centrifuge carriers. Centrifuge specimen for ≤5 minutes, then promptly transfer plasma.)

5. Immediately freeze plasma.

Additional Information: See Renin-Aldosterone Studies in Special Instructions for further information.


UChicago Medicine Medical Laboratories Note:

Offsite Collection Instructions:

 

Spin Lavender top

Aliquot plasma

Place in the freezer until courier pick up

Send frozen sample on dry ice to the Main Lab (Laboratory Service Center)

Specimen Minimum Volume

1.15 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Plasma EDTA Frozen 14 days

Special Instructions

Reference Values

0-2 years: 4.6 ng/mL/hour (mean)* Range: 1.4-7.8 ng/mL/hour

3-5 years: 2.5 ng/mL/hour (mean)* Range: 1.5-3.5 ng/mL/hour

6-8 years: 1.4 ng/mL/hour (mean)* Range: 0.8-2.0 ng/mL/hour

9-11 years: 1.9 ng/mL/hour (mean)* Range: 0.9-2.9 ng/mL/hour

12-17 years: 1.8 ng/mL/hour (mean)* Range: 1.2-2.4 ng/mL/hour

Mean data not standardized as to time of day or diet. Infants were supine, children sitting.

 

Na-depleted, upright (peripheral vein specimen)

18-39 years: 10.8 ng/mL/hour (mean)

2.9-24.0 ng/mL/hour (range)

≥40 years: 5.9 ng/mL/hour (mean)

2.9-10.8 ng/mL/hour (range)

 

Na-replete, upright (peripheral vein specimen)

18-39 years: 1.9 ng/mL/hour (mean)

≤0.6-4.3 ng/mL/hour (range)

≥40 years: 1.0 ng/mL/hour (mean)

≤0.6-3.0 ng/mL/hour (range)

*Stalker HP, Holland NH, Kotchen JM, Kotchen TA: Plasma renin activity in healthy children. J Pediatr 1976;89:256-258

Day(s) and Time(s) Performed

Monday through Friday; 1 p.m.

CPT Code Information

84244

Reject Due To

Gross hemolysis Reject
Gross lipemia OK
Gross icterus OK

Method Name

Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)

Portions of this test are covered by patents held by Quest Diagnostics

Forms

If not ordering electronically, complete, print, and send 1 of the following forms with the specimen:

-General Request (T239)

-Cardiovascular Test Request (T724)