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The University of Chicago Medicine Laboratories

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Specimen Collection and Handling

Transfusion

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Mayo Clinic Laboratories

Test Code PFA PFA-100 (Platelet Function Analyzer)

Specimen and Container/Tube

Specimen Type: Whole Blood

Preferred Specimen: Light Blue Top Tube, 3.2% Sodium Citrate

Other Acceptable specimens: N/A

Volume

Preferred Volume: Two Blue Top Tubes, 3.2% Sodium Citrate

 

Minimum Volume: Two Blue Top Tubes, 3.2% Sodium Citrate

Specimen Stability

Storage Temperature

Stability

Room temperature

4 hours

Refrigerated

not acceptable

Frozen

not acceptable

Collection Instructions

Collect specimen per standard laboratory collection procedures. 

 

NOTE: Once each blue top tube is completely filled, gently invert the sample tube at least five times to ensure complete mixing of the contents.

Specimen Handling and Transport Instructions

UCMC Onsite Instructions: 

Collect specimen per standard collection procedure and send to laboratory immediately. 

 

NOTE: Once each blue top tube is completely filled, gently invert the sample tube at least five times to ensure complete mixing of the contents.

 

UCMC Offsite and UC MedLab Outreach Instructions: 

  • Whole blood sample must arrive in the laboratory within three hours of specimen collection.
  • Draw two blue top tubes.
  • Once each blue top tube is completely filled, gently invert the sample tube at least five times to ensure complete mixing of the contents.
  • Do not centrifuge samples. Centrifuged samples will be rejected.
  • Send samples Room Temperature. Refrigerated or frozen samples will be rejected. 

Rejection Criteria

  • Common specimen rejected situations include: incorrect specimen type, insufficient volume, missing or incomplete specimen identifiers, incorrect specimen transport, or specimens outside stability limits. 
  • Specimens that have wrong ratio of blood to anticoagulant (under/overfilled), clotted, heated, refrigerated, frozen, or centrifuged (reconstitution of a centrifuged specimen by mixing is not acceptable).

Reference Values

 

Test

Age

Sex

Reference Range

Collagen/Epinephrine

ALL

ALL

80 - 190 seconds

Collagen/ADP

ALL

ALL

60 - 122 seconds

Critical Values

Analyte

Unit

Low

High

Age

N/A

N/A

N/A

N/A

N/A

Turnaround Time

Turnaround times are relative to the time the specimen is received in the test laboratory. 

STAT Turnaround Time: N/A

 

Routine Turnaround Time: 4 hours

 

Testing Schedule: 24/7

Clinical Indications

Screening for disorders of primary hemostasis (von Willebrand disease, platelet dysfunction), either congenital or acquired

Test Components

Collagen/Epinephrine

Collagen/ADP

Methodology

Platelet aggregation

CPT Codes

85576 X2

LOINC Codes

24471-5, 24472-3

Synonyms/Keyword Search

PFA-100, Epi, ADP, Epinephrine, Primary hemostasis screen

Additional Information

The test should not be ordered if hematocrit is below 30% and/or platelet count is below 100,000/uL.

 

3.2% sodium citrate light blue top tubes must have 9 to 1 ratio of blood to anticoagulant

Last Review Date

04/01/2025