Test Code NPM NPM1 Mutation Analysis (Blood)

Important Note

Starting on 05/22/2017, the University of Chicago Molecular Pathology Laboratory launched a new NGS assay for the detection of mutations in Exon 11 of NPM1 gene. This test replaces the previous fragment analysis based qualitative assay and has a much improved analytical sensitivity (0.2% vs 5%).

Additional Codes

n/a

Specimen and Container/Tube

Blood

Rejection Criteria

Inappropriate anticoagulant and >72 hours

Turnaround Time

Not Available

Test Includes

Detects NPM1 insertion mutations in Exon 11

Specimen Type

Blood

Preferred Volume

3 mL

Test Usage

May be used to monitor low level residual disease in acute
myeloid leukemia (AML) patients.

Synonyms

NPM

Offsite Collection Instructions

Send within 24 hours or Refrigerate/ send within 72 hours

UCMC Collection Instructions

Send within 72 hours

Test Limitations

Copy number alterations will not be detected. Assay
sensitivity
is 0.2% MAF
(one mutant molecule in a
background of 500 normal molecules).

Fee Code

376540-30081310

STAT Turnaround Time

Not Available

Container/Tube

Lavender top

Specimen Minimum Volume

1 mL

Special Instructions

Send within 72 hours

Day(s) Performed

Monday – Friday, 8:00 AM – 4:30 PM

STAT DAY(S) AND TIME(S) PERFORMED

CPT

81310

Method Name

Next Generation Sequencing

Reference Values

Interpretative report

Reject Due To