Test Code MAFP1 Alpha-Fetoprotein (AFP), Single Marker Screen, Maternal, Serum
Necessary Information
In order to provide the best results, either answer the order entry questions or provide the required information using the Second Trimester Maternal Screening Alpha-Fetoprotein / Quad Screen Patient Information (T595).
Specimen Required
Collection Container/Tube:
Preferred: Serum gel
Acceptable: Red top
Submission Container/Tube: Plastic vial
Specimen Volume: 1 mL
Collection Instructions:
1. Do not collect specimen after amniocentesis as this could affect results.
2. Within 2 hours of collection, centrifuge and aliquot serum into a plastic vial.
Additional Information:
1. Collect blood between 15 weeks, 0 days and 22 weeks, 6 days.
2. Initial or repeat testing is determined in the laboratory at the time of report and will be reported accordingly. To be considered a repeat test for the patient, the testing must be within the same pregnancy and trimester, with interpretable results for the same test, and both tests are performed at Mayo Clinic.
Forms
1. If not ordering electronically, Second Trimester Maternal Screening Alpha-Fetoprotein / Quad Screen Patient Information (T595) is required.
2. If not ordering electronically, complete, print, and send a General Request (T239).
Secondary ID
113382Special Instructions
Method Name
Two-Site Immunoenzymatic (Sandwich) Assay
Specimen Type
SerumSpecimen Minimum Volume
0.5 mL
Specimen Stability Information
Specimen Type | Temperature | Time |
---|---|---|
Serum | Refrigerated (preferred) | 14 days |
Frozen | 90 days | |
Ambient | 7 days |
Reject Due To
Gross hemolysis | Reject |
Gross lipemia | OK |
Gross icterus | OK |
Reference Values
An alpha-fetoprotein (AFP) multiple of the median (MoM) <2.5 is reported as screen negative.
AFP MoM ≥2.5 (singleton and twin pregnancies) are reported as screen positive.
An interpretive report will be provided.
Day(s) Performed
Monday through Friday
Report Available
4 to 6 daysPerforming Laboratory

CPT Code Information
82105