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HelpTest Code LS6 Lymphocyte Subset 6
Specimen and Container/Tube
Specimen Type: Whole blood
Preferred Specimen: lavender top
Other Acceptable specimens: not applicable
Collection Instructions
Collect specimen per standard laboratory collection procedures.
Specimen Handling and Transport Instructions
UCMC Onsite Instructions: Collect specimen per standard collection procedure and send to laboratory immediately.
UCMC Offsite and UC MedLab Outreach Instructions: Collect specimen per standard collection procedure and send to laboratory immediately. Specimens must be received within 24 hours. If transport is delayed, retain specimen at room temperature.
Reference Values
|
Analyte |
Age |
Sex |
Reference Range |
|
Absolute lymphocyte count |
3 months |
Male and female |
3400 – 7600 |
|
Absolute lymphocyte count |
6 months |
Male and female |
3900 – 9000 |
|
Absolute lymphocyte count |
1 year |
Male and female |
3400 – 9000 |
|
Absolute lymphocyte count |
2 years |
Male and female |
3600 – 8900 |
|
Absolute lymphocyte count |
6 years |
Male and female |
2300 – 5400 |
|
Absolute lymphocyte count |
12 years |
Male and female |
1900 – 3700 |
|
Absolute lymphocyte count |
12 years and up |
Male and female |
1200 – 3265 |
|
CD3 percent |
3 months |
Male and female |
53 – 84 |
|
CD3 percent |
6 months |
Male and female |
51 – 77 |
|
CD3 percent |
1 year |
Male and female |
49 – 76 |
|
CD3 percent |
2 years |
Male and female |
53 – 75 |
|
CD3 percent |
6 years |
Male and female |
56 – 75 |
|
CD3 percent |
12 years |
Male and female |
60 – 76 |
|
CD3 percent |
12 years and up |
Male and female |
61 – 82 |
|
CD3 absolute count |
3 months |
Male and female |
2500 – 5500 |
|
CD3 absolute count |
6 months |
Male and female |
2500 – 5600 |
|
CD3 absolute count |
1 year |
Male and female |
1900 – 5900 |
|
CD3 absolute count |
2 years |
Male and female |
2100 – 6200 |
|
CD3 absolute count |
6 years |
Male and female |
1400 – 3700 |
|
CD3 absolute count |
12 years |
Male and female |
1200 – 2600 |
|
CD3 absolute count |
12 years and up |
Male and female |
828 – 2328 |
|
CD4 percent |
3 months |
Male and female |
35 – 64 |
|
CD4 percent |
6 months |
Male and female |
35 – 56 |
|
CD4 percent |
1 year |
Male and female |
31 – 56 |
|
CD4 percent |
2 years |
Male and female |
32 – 51 |
|
CD4 percent |
6 years |
Male and female |
28 – 47 |
|
CD4 percent |
12 years |
Male and female |
31 - 47 |
|
CD4 percent |
12 years and up |
Male and female |
35 – 60 |
|
CD4 absolute count |
3 months |
Male and female |
1600 – 4000 |
|
CD4 absolute count |
6 months |
Male and female |
1800 – 4000 |
|
CD4 absolute count |
1 year |
Male and female |
1400 – 4300 |
|
CD4 absolute count |
2 years |
Male and female |
1300 – 4300 |
|
CD4 absolute count |
6 years |
Male and female |
700 – 2220 |
|
CD4 absolute count |
12 years |
Male and female |
650 – 1500 |
|
CD4 absolute count |
12 years and up |
Male and female |
515 – 1642 |
|
CD8 percent |
3 months |
Male and female |
12 – 28 |
|
CD8 percent |
6 months |
Male and female |
12 – 23 |
|
CD8 percent |
1 year |
Male and female |
12 – 24 |
|
CD8 percent |
2 years |
Male and female |
14 – 30 |
|
CD8 percent |
6 years |
Male and female |
16 – 30 |
|
CD8 percent |
12 years |
Male and female |
18 – 35 |
|
CD8 percent |
12 years and up |
Male and female |
14 – 38 |
|
CD8 absolute count |
3 months |
Male and female |
560 – 1700 |
|
CD8 absolute count |
6 months |
Male and female |
590 – 1600 |
|
CD8 absolute count |
1 year |
Male and female |
500 – 1700 |
|
CD8 absolute count |
2 years |
Male and female |
620 – 2000 |
|
CD8 absolute count |
6 years |
Male and female |
490 – 1300 |
|
CD8 absolute count |
12 years |
Male and female |
370 – 1100 |
|
CD8 absolute count |
12 years and up |
Male and female |
212 – 887 |
|
CD4:CD8 Ratio |
3 months |
Male and female |
1.3 – 5.3 |
|
CD4:CD8 Ratio |
6 months |
Male and female |
1.5 – 4.7 |
|
CD4:CD8 Ratio |
1 year |
Male and female |
1.3 – 4.7 |
|
CD4:CD8 Ratio |
2 years |
Male and female |
1.1 – 3.6 |
|
CD4:CD8 Ratio |
6 years |
Male and female |
0.9 – 2.9 |
|
CD4:CD8 Ratio |
12 years |
Male and female |
0.9 – 4.3 |
|
CD4:CD8 Ratio |
12 years and up |
Male and female |
0.9 – 3.3 |
Turnaround Time
Turnaround times are relative to the time the specimen is received in the test laboratory.
STAT Turnaround Time: not available
Routine Turnaround Time: 24 hours
Testing Schedule: Monday - Friday 8:00 am - 5:00 pm and Saturday 8:00 am - 3:00 pm
Volume
Preferred Volume: 4.0 mL
Minimum Volume: 1.0 mL
Specimen Stability
|
Storage Temperature |
Stability |
|
Room temperature (20 - 25°C) |
24 hours |
|
Refrigerated (2 - 10°C) |
not acceptable |
|
Frozen (-20°C) |
not acceptable |
Rejection Criteria
- Common specimen rejected situations include: incorrect specimen type, insufficient volume, missing or incomplete specimen identifiers, incorrect specimen transport, or specimens outside stability limits.
- Frozen or heated specimens will be rejected
- Clotted or quantity not sufficient specimens are not suitable for testing and will be rejected
Critical Values
not applicable
Test Components
Absolute lymphocyte count, CD3 percent, CD3 absolute count, CD4 percent, CD4 absolute count, CD8 percent, CD8 absolute count, CD4:CD8 ratio, CD45+CD14+ percent, CD45+CD14+ absolute count, CD3+CD27-CD28- percent, CD3+CD27+CD28- absolute count
Clinical Indications
- T cell exhaustion/Asthenia
Methodology
Flow cytometry
CPT Codes
86355, 86356 x6, 86359
LOINC Codes
ABLY 30364-4, CD 33719-6
Synonyms/Search Keywords
T cell exhaustion/Asthenia
Last Reviewed Date
8/12/2024