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Test Code LS6 Lymphocyte Subset 6

Specimen and Container/Tube

Specimen Type: Whole blood

Preferred Specimen: lavender top

Other Acceptable specimens: not applicable 

Specimen Handling and Transport Instructions

UCMC Onsite Instructions: Collect specimen per standard collection procedure and send to laboratory immediately.

UCMC Offsite and UC MedLab Outreach Instructions: Collect specimen per standard collection procedure and send to laboratory immediately. Specimens must be received within 24 hours. If transport is delayed, retain specimen at room temperature. 

Rejection Criteria

  • Common specimen rejected situations include: incorrect specimen type, insufficient volume, missing or incomplete specimen identifiers, incorrect specimen transport, or specimens outside stability limits. 
  • Frozen or heated specimens will be rejected
  • Clotted or quantity not sufficient specimens are not suitable for testing and will be rejected 

 

Reference Values

Analyte

Age

Sex

Reference Range

Absolute lymphocyte count

3 months

Male and female

3400 – 7600

Absolute lymphocyte count

6 months

Male and female

3900 – 9000

Absolute lymphocyte count

1 year

Male and female

3400 – 9000

Absolute lymphocyte count

2 years

Male and female

3600 – 8900

Absolute lymphocyte count

6 years

Male and female

2300 – 5400

Absolute lymphocyte count

12 years

Male and female

1900 – 3700

Absolute lymphocyte count

12 years and up

Male and female

1200 – 3265

CD3 percent

3 months

Male and female

53 – 84

CD3 percent

6 months

Male and female

51 – 77

CD3 percent

1 year

Male and female

49 – 76

CD3 percent

2 years

Male and female

53 – 75

CD3 percent

6 years

Male and female

56 – 75

CD3 percent

12 years

Male and female

60 – 76

CD3 percent

12 years and up

Male and female

61 – 82

CD3 absolute count

3 months

Male and female

2500 – 5500

CD3 absolute count

6 months

Male and female

2500 – 5600

CD3 absolute count

1 year

Male and female

1900 – 5900

CD3 absolute count

2 years

Male and female

2100 – 6200

CD3 absolute count

6 years

Male and female

1400 – 3700

CD3 absolute count

12 years

Male and female

1200 – 2600

CD3 absolute count

12 years and up

Male and female

828 – 2328

CD4 percent

3 months

Male and female

35 – 64

CD4 percent

6 months

Male and female

35 – 56

CD4 percent

1 year

Male and female

31 – 56

CD4 percent

2 years

Male and female

32 – 51

CD4 percent

6 years

Male and female

28 – 47

CD4 percent

12 years

Male and female

31 - 47

CD4 percent

12 years and up

Male and female

35 – 60

CD4 absolute count

3 months

Male and female

1600 – 4000

CD4 absolute count

6 months

Male and female

1800 – 4000

CD4 absolute count

1 year

Male and female

1400 – 4300

CD4 absolute count

2 years

Male and female

1300 – 4300

CD4 absolute count

6 years

Male and female

700 – 2220

CD4 absolute count

12 years

Male and female

650 – 1500

CD4 absolute count

12 years and up

Male and female

515 – 1642

CD8 percent

3 months

Male and female

12 – 28

CD8 percent

6 months

Male and female

12 – 23

CD8 percent

1 year

Male and female

12 – 24

CD8 percent

2 years

Male and female

14 – 30

CD8 percent

6 years

Male and female

16 – 30

CD8 percent

12 years

Male and female

18 – 35

CD8 percent

12 years and up

Male and female

14 – 38

CD8 absolute count

3 months

Male and female

560 – 1700

CD8 absolute count

6 months

Male and female

590 – 1600

CD8 absolute count

1 year

Male and female

500 – 1700

CD8 absolute count

2 years

Male and female

620 – 2000

CD8 absolute count

6 years

Male and female

490 – 1300

CD8 absolute count

12 years

Male and female

370 – 1100

CD8 absolute count

12 years and up

Male and female

212 – 887

CD4:CD8 Ratio

3 months

Male and female

1.3 – 5.3

CD4:CD8 Ratio

6 months

Male and female

1.5 – 4.7

CD4:CD8 Ratio

1 year

Male and female

1.3 – 4.7

CD4:CD8 Ratio

2 years

Male and female

1.1 – 3.6

CD4:CD8 Ratio

6 years

Male and female

0.9 – 2.9

CD4:CD8 Ratio

12 years

Male and female

0.9 – 4.3

CD4:CD8 Ratio

12 years and up

Male and female

0.9 – 3.3

Critical Values

not applicable

Turnaround Time

Turnaround times are relative to the time the specimen is received in the test laboratory. 

 

STAT Turnaround Time: not available 

 

Routine Turnaround Time: 24 hours

 

Testing Schedule: Monday - Friday 8:00 am - 5:00 pm and Saturday 8:00 am - 3:00 pm

CPT Codes

86355, 86356 x6,  86359

LOINC Codes

ABLY 30364-4, CD 33719-6 

Volume

Preferred Volume: 4.0 mL

Minimum Volume: 1.0 mL

Specimen Stability

Storage Temperature

Stability

Room temperature

(20 - 25°C)

 24 hours 

Refrigerated

(2 - 10°C)

 not acceptable

Frozen

(-20°C)

not acceptable

Collection Instructions

Collect specimen per standard laboratory collection procedures. 

Test Components

Absolute lymphocyte count, CD3 percent, CD3 absolute count, CD4 percent, CD4 absolute count, CD8 percent, CD8 absolute count, CD4:CD8 ratio, CD45+CD14+ percent, CD45+CD14+ absolute count, CD3+CD27-CD28- percent, CD3+CD27+CD28- absolute count

Clinical Indications

  • T cell exhaustion/Asthenia 

Methodology

Flow cytometry

Synonyms/Search Keywords

T cell exhaustion/Asthenia 

Last Reviewed Date

8/12/2024