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The University of Chicago Medicine Laboratories

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Mayo Clinic Laboratories

Test Code LABTXXMAU (Test code: 858044) Transplant Crossmatch: Recipient Auto-crossmatch

Ordering Notes

For crossmatch testing, an order for recipient HLA Antibody Testing is needed.

For STAT turn-around time, please send a request to the Transplant Immunology Laboratory by e-mail at HLA-TIILLab@uchicagomedicine.org and/or by pager #8722.

Specimen and Container/Tube

Specimen Type: Whole blood and serum
Preferred Specimen: ACD solution A (2) and gold top SST (2)
Other Acceptable Specimens: For serum only: Tiger top (marble) SST, red top

Volume

Preferred Volume: 20mL whole blood (ACD), 6mL (serum)
Minimum Volume: 20mL whole blood (ACD), 6mL (serum)

Specimen Stability

Whole blood specimens may be kept at room temperature for up to 72 hours following collection.

Serum specimens may be kept at room temperature for up to 24 hours following collection. Once centrifuged, specimens may be kept at refrigeraton (4°C) for up to one week.

Collection Instructions

Collect specimen per standard laboratory collection procedures.

Specimen Handling and Transport Instructions

UCM Onsite Instructions: Collect specimen per standard collection procedures and send to the laboratory immediately.
UCM Offsite and UC MedLab Outreach Instructions: Collect specimen per standard collection procedures and send to the laboratory using standard transport procedures.

Rejection Criteria

Whole blood specimens greater than 72 hours old may be rejected; cellular viability may not be adequate for testing.

Unspun serum samples greater than 24 hours may be rejected. Sample with visible signs of hemolysis (H-Index > 100) may be rejected.

Reference Values

No reference range; results may be provided in an interpretive report.

Critical Values

Not Applicable

Turnaround Time

Turnaround times are relative to the time the specimen is received in the testing laboratory section.

STAT Turnaround Time: 2 business days
Routine Turnaround Time: 4 business days

If test results are needed same-day, please contact the laboratory at 2-0700, and page #8722.

Testing Schedule: M-F 7:00am-7:00pm

Clinical Indications

The flow cytometric crossmatch is a test that assesses the level of circulating antibodies in the recipient that are directed against the HLA class I and/or class II molecules of the recipient cells (self). This test may determine if a recipient’s autoantibodies are responsible for a crossmatch result, and can be used to troubleshoot unexpected reactivity from other methods.

Test Components

T- and B-cell crossmatch

CPT Codes

Flow crossmatch, initial serum sample: 86825

Flow crossmatch, additional serum sample or dilution: 86826

Methodology

Flow cytometry

Synonyms/Key Search Words

Crossmatch, Transplant, HLA

Last Review Date

10/16/2024