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HelpTest Code LABMDONCOSCRN OncoScreen PBBM
Specimen and Container/Tube
Specimen Type: Peripheral Blood / Bone Marrow Aspirates
Preferred Specimen: lavendar top (EDTA).
Other Acceptable Specimens: N/A
Specimen Stability
Blood and bone marrow aspirate specimens may be kept at room temperature for up to 6 hours following collection, or at 4°C for up to two weeks.
Collection Instructions
Collect specimen per standard laboratory collection procedures.
Specimen Handling and Transport Instructions
UCM Onsite Instructions: Collect specimen per standard collection procedured and send to the laboratory immediately.
Clinical Indications
Targeted and rapid assay covering hotspot regions in 50 cancer associated genes for genomic profiling of solid tumor and hematological malignancy specimens to determine appropriate therapies. It also can be useful for diagnosis, prognosis, clinical trials selection and for translational research.
CPT Codes
81450
Methodology
Next generation sequencing
Additional Information
Detects hotspot regions in 50 genes that are commonly mutated in cancer: ABL1, AKT1, ALK, APC, ATM, BRAF, CDH1, CDKN2A, CSF1R, CTNNB1, EGFR, ERBB2, ERBB4, EZH2, FBXW7, FGFR1, FGFR2, FGFR3, FLT3, GNA11, GNAQ, GNAS, HNF1A, HRAS, IDH1, IDH2, JAK2, JAK3, KDR, KIT, KRAS, MET, MLH1, MPL, NOTCH1, NPM1, NRAS, PDGFRA, PIK3CA, PTEN, PTPN11, RB1, RET, SMAD4, SMARCB1, SMO, SRC, STK11, TP53, VHL. Will not detect mutations below the analytical sensitivity of the assay (5% variant allelic frequency).
Ordering Notes
For questions about Molecular Pathology orders, email MolecularDxLab@uchicagomedicine.org
Volume
Preferred Volume: 3mL
Minimum Volume: 3mL
Turnaround Time
Turnaround times are relative to the time the specimen is received in the testing laboratory section.
STAT Turnaround Time: n/a
Routine Turnaround Time: ≤10 days
Testing Schedule: M-F 7:00am-5:00pm
Infectious Specimen
N/A
Search Keywords
Hotspot panel, cancer, mutation, NGS, NextGen, Sequencing, Gene