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The University of Chicago Medicine Laboratories

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Mayo Clinic Laboratories

Test Code LABCCFISH CANCER CYTOGENETICS, FISH

Specimen and Container/Tube

UCMC Onsite Instructions
Bone Marrow/Aspirate/Core: Please call the Hematopathology Laboratory (x2-1314) for custom made collection tubes
Peripheral Blood: Sodium Heparin (NaHep, dark green top) vacutainer
CSF & non-CSF Fluid: Sterile collection cup or conical tube without additives
Lymph Node/Mass: Please call the Hematopathology Laboratory (x2-1314) for custom made collection tubes

UCMC Offsite and UC MedLab Outreach Instructions
Bone Marrow/Aspirate/Core: Please call the Cytogenetics Laboratory (773-702-6203) for custom made collection tubes
Peripheral Blood: Sodium Heparin (NaHep, dark green top) vacutainer
CSF & non-CSF Fluid: Sterile collection cup or conical tube without additives
Lymph Node/Mass: Please call the Cytogenetics Laboratory (773-702-6203) for custom make collection tubes

Volume

Bone Marrow/Aspirate: >3 mL
Bone Core: >5 mm
Peripheral Blood: >3 mL
CSF & non-CSF Fluid: >3 mL
Lymph Node/Mass: >5 mm

Specimen Stability

Specimens are stable at room temperature up to 24 hours.

Note: Prolonged transport to the laboratory diminishes success of tumor cell viability in culture.

Specimen Handling and Transport Instructions

UCMC Onsite Instructions
Transport specimen at room temperature, do not freeze or refrigerate.

Specimen should be received by the cytogenetics laboratory <24 hours post collection.

Peripheral blood samples can be sent directly to Cytogenetics via tube station #345

UC MedLab Outreach institutions please contact Pathology Client Services (773-702-8287) for transport instructions.

Rejection Criteria

Specimens exposed to fixatives

Reference Values

Laboratory established reference values or published guidelines for interpretation (eg ASCO/CAP) will be included on the report.

Results reported with current recommendations of the International Standing Committee on Human Cytogenomic Nomenclature (ISCN).

Clinical Indications

Myeloid and lymphoid neoplasms

Test Components

Tissue culture
cell harvesting
slide preparation
fluorescence in situ hybridization (FISH) with enumeration, dual fusion break apart, dual color dual fusion, tri color, and dual color single fusion probes; interpretive report

Methodology

Fluorescence in situ hybridization (FISH)

CPT Codes

88237
88239
88271
88275

Additional Information

Limitations: Will not detect abnormalities of genes, loci, or chromosomes not specifically targeted

Probes Offered:
CEP X/Y D5S72,D5S23/EGR1 [-5/del(5q)]
BCR::ABL1 [t(9;22)]
CBFB [inv(16)/t(16;16]
D20S108 [del(20q)]
D7S486/D7Z1 [-7/del(7q)]
KMT2A [11q23]
FIL1L1::PDGFRA [4q12]
PDGFRB [5q32q33]
PML::RARA [t(15;17)]
RUNX1::RUNX1T1 [t(8;21)]
CEP8 [gains/losses of chromosome 8]
EVI1 [inv(3)/t(3;3)]
FGFR1 [8p11.2], CEP8
FLT3 [13q12.2]
NUP98 [11p15.4]
CEP4 [gains/losses of chromosome 4]
CEP10 [gains/losses of chromosome 10]
CEP17 [gains/losses of chromosome 17]
ETV6::RUNX1 [t(12;21)]
IGH [14q32]
CRLF2 [Xp22.33/Yp11.32]
ABL2 [1q25.2]
ABL1 [9q34.11q34.13]
CSF1R [5q32]
JAK2 [9p24]
CEP X/Y
MYC [8q24]
IGH::MYC [t(8;14)], CEP 8
IGH::BCL2 [t(14;18)]
BCL6 [3q27]
IGH::CCND1 [t(11;14)]
ATM [11q22.3]
TP53 [17p13.1]
CEP12 [gains/losses of chromosome 12]
D13S319/LAMP1 [-13/del(13q)]
IGH::FGFR3 [t(4;14)]
IGH::CCND1 [t(11;14)]
IGH::MAF [t(14;16)]
CDKN2C [1p32.3]/CKS1B [1q21]
CEP3 [gains/losses of chromosome 3]
CEP7 [gains/losses of chromosome 7]
CEP9 [gains/losses of chromosome 9]
CBFB::MYH11
Panels Offered:
MDS Panel: -5/del(5q), -7/del(7q), CEP8, del(20q)
Ped B-ALL Panel: CEP4/10/17, ETV6::RUNX1, BCR::ABL1, MLL
Ph-like ALL Panel: BCR::ABL1, IGH, CRLF2, JAK2, CSF1R, ABL1, ABL2, PDGFRB
Ped T-ALL Panel: BCR::ABL1, MLL, ABL1
MM Panel: IGH, IGH::FGFR3, 1p/1q, CEP3/7/9, ATM, TP53, CEP12, -13/del(13q)
*If IGH is positive and IGH::FGFR3 is negative IGH::CCND1 and IGH::MAF is reflexed
CLL Panel: IGH, ATM, TP53, CEP12, -13/del(13q), IGH::CCND1
AML Panel: -5/del(5q), -7/del(7q), EVI1, MLL, RUNX1::RUNX1T1, CBFB
DLBCL/HGBCL Panel: MYC, IGH::MYC, BCL6, IGH::BCL2
Eosinophilia Panel: FIP1L1::PDGFRA, PDGFRB, JAK2, FGFR1, FLT3

Last Review Date

10/9/2024

Specimen & Container/Tube

UCMC Onsite Instructions
Bone Marrow/Aspirate/Core: Please call the Hematopathology Laboratory (x2-1314) for custom made collection tubes
Peripheral Blood: Sodium Heparin (NaHep, dark green top) vacutainer
CSF & non-CSF Fluid: Sterile collection cup or conical tube without additives
Lymph Node/Mass: Please call the Hematopathology Laboratory (x2-1314) for custom made collection tubes

 

UCMC Offsite and UC MedLab Outreach Instructions
Bone Marrow/Aspirate/Core: Please call the Cytogenetics Laboratory (773-702-9153) for custom made collection tubes
Peripheral Blood: Sodium Heparin (NaHep, dark green top) vacutainer
CSF & non-CSF Fluid: Sterile collection cup or conical tube without additives
Lymph Node/Mass: Please call the Cytogenetics Laboratory (773-702-9153) for custom made collection tubes

Specimen Handling & Transport Instructions

Transport specimen at room temperature, do not freeze or refrigerate
Specimen should be received by the cytogenetics laboratory <24 hours post collection

For UC MedLab Outreach institutions please contact Pathology Client Services (773-702-8287) for transport instructions

Synonyms/ Search Keyword

FISH

Ordering Notes

For questions about FISH probes email #UCM-Cytogenetics-Lab, or CytogeneticsLab@uchicagomedicine.org

Turnaround Time

up to 7 days

Please note: Turnaround times can vary based on lab staffing. For urgent needs and requests to expedite testing, please email #UCM-Cytogenetics-Lab, or CytogeneticsLab@uchicagomedicine.org

Synonyms/Key Search Words

FISH