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Test Code LABCCFISH FISH, CANCER CYTOGENETICS

Rejection Criteria

Specimens exposed to fixatives

Reference Values

Interpretative Report

CPT Codes

88237, 88239, 88271, 88275

Specimen & Container/Tube

UCMC Onsite Instructions
Bone Marrow/Aspirate/Core: Please call the Hematopathology Laboratory (x2-1314) for custom made collection tubes
Peripheral Blood: Sodium Heparin (NaHep, dark green top) vacutainer
CSF & non-CSF Fluid: Sterile collection cup or conical tube without additives
Lymph Node/Mass: Please call the Hematopathology Laboratory (x2-1314) for custom made collection tubes

 

UCMC Offsite and UC MedLab Outreach Instructions
Bone Marrow/Aspirate/Core: Please call the Cytogenetics Laboratory (773-702-9153) for custom made collection tubes
Peripheral Blood: Sodium Heparin (NaHep, dark green top) vacutainer
CSF & non-CSF Fluid: Sterile collection cup or conical tube without additives
Lymph Node/Mass: Please call the Cytogenetics Laboratory (773-702-9153) for custom made collection tubes

Volume

Bone Marrow/Aspirate: >3 mL
Bone Core: >5 mm
Peripheral Blood: >3 mL
CSF & non-CSF Fluid: >3 mL
Lymph Node/Mass: >5 mm

Specimen Handling & Transport Instructions

Transport specimen at room temperature, do not freeze or refrigerate
Specimen should be received by the cytogenetics laboratory <24 hours post collection

For UC MedLab Outreach institutions please contact Pathology Client Services (773-702-8287) for transport instructions

Clinical Indications

Myeloid and lymphoid neoplasms

Test Components

Tissue culture, cell harvesting, slide preparation, FISH analysis

Methodology

Tissue culture and fluorescence in situ hybridization (FISH) with enumeration, dual fusion break apart, dual color dual fusion, tri color, and dual color single fusion probes

Synonyms/ Search Keyword

FISH

Additional Information

Limitations: Will not detect abnormalities of genes, loci, or chromosomes not specifically targeted

Probes Offered:
D5S72,D5S23/EGR1 [-5/del(5q)]
BCR::ABL1 [t(9;22)]
CBFB [inv(16)/t(16;16]
D20S108 [del(20q)]
D7S486/D7Z1 [-7/del(7q)]
KMT2A [11q23]
FIL1L1::PDGFRA [4q12]
PDGFRB [5q32]
PML::RARA [t(15;17)]
RUNX1::RUNX1T1 [t(8;21)]
CEP8 [gains/losses of chromosome 8]
EVI1 [inv(3)/t(3;3)]
FGFR1 [8p11.2], CEP8
FLT3 [13q12.2]
NUP98 [11p15.4]
CEP4 [gains/losses of chromosome 4]
CEP10 [gains/losses of chromosome 10]
CEP17 [gains/losses of chromosome 17]
ETV6::RUNX1 [t(12;21)]
IGH [14q32]
CRLF2 [Xp22.33/Yp11.32]
ABL2 [1q25.2]
ABL1 [9q34.11q34.13]
CSF1R [5q32]
JAK2 [9p24]
PDGFRB [5q32q33]
MYC [8q24]
IGH::MYC [t(8;14)], CEP 8
IGH::BCL2 [t(14;18)]
BCL6 [3q27]
IGH::CCND1 [t(11;14)]
ATM [11q22.3]
TP53 [17p13.1]
CEP12 [gains/losses of chromosome 12]
D13S319/LAMP1 [-13/del(13q)]
IGH::FGFR3 [t(4;14)]
IGH::CCND1 [t(11;14)]
IGH::MAF [t(14;16)]
CDKN2C [1p32.3]/CKS1B [1q21]
CEP3 [gains/losses of chromosome 3]
CEP7 [gains/losses of chromosome 7]
CEP9 [gains/losses of chromosome 9]

Panels Offered:
MDS Panel: -5/del(5q), -7/del(7q), CEP8, del(20q)
Ped B-ALL Panel: CEP4/10/17, ETV6::RUNX1, BCR::ABL1, MLL
Ph-like ALL Panel: BCR::ABL1, IGH, CRLF2, JAK2, CSF1R, ABL1, ABL2, PDGFRB
Ped T-ALL Panel: BCR::ABL1, MLL, ABL1
MM Panel: IGH, IGH::FGFR3, 1p/1q, CEP3/7/9, ATM, TP53, CEP12, -13/del(13q)
*If IGH is positive and IGH::FGFR3 is negative IGH::CCND1 and IGH::MAF is reflexed
CLL Panel: IGH, ATM, TP53, CEP12, -13/del(13q)
AML Panel: -5/del(5q), -7/del(7q), EVI1, MLL, RUNX1::RUNX1T1, CBFB
DLBCL/HGBCL Panel: MYC, IGH::MYC, BCL6, IGH::BCL2
Eosinophilia Panel: FIP1L1::PDGFRA, PDGFRB, JAK2, FGFR1, FLT3

Last Review Date

April 2023