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Test Code IGNC Culture, Genital, Female (Internal), Bacteria

Specimen and Container/Tube

Swab, fluid, or aspirate Site: Endometrium, placenta, Bartholin gland, amniotic fluid

Anaerobic culture is recommended for amniotic fluid. See Anaerobe/Aerobe culture (ANAC).

Transport swab, sterile container, or rubber-capped syringe. See ‘Additional Information’ for preferred volumes

Specimen Handling and Transport Instructions

Genital Site Volume Transport
Endometrium Currettings or aspirate preferred; 2 swabs
acceptable		 Rubber-capped syringe, Sterile container, Transport Swab
Placenta Tissue or aspirate preferred; 2 swabs acceptable Rubber-capped syringe, Sterile container, Transport Swab
Bartholin gland Exudate, 0.5 mL Rubber-capped syringe or Transport Swab
Amniotic fluid Uncontaminated fluid?�10-15 mL preferred;?�8 mL
minimum		 Sterile container

Rejection Criteria

Whole blood/unspun samples greater than 6 hours will be rejected.

Reference Values

Negative

Critical Values

 

All positive  culture

 

 

 

 

All

Turnaround Time

Gram stain only: within 1 hr of receipt in Microbiology lab, if STAT requested.

STAT Turnaround Time

Gram stain only: within 1 hr of receipt in Microbiology lab, if STAT requested.

Day(s) Performed

24/7

STAT DAY(S) AND TIME(S) PERFORMED

Available

CPT Codes

87070, 87205

LOINC Codes

30887070, 30687205

Volume

Genital Site Volume Transport
Endometrium Currettings or aspirate preferred; 2 swabs
acceptable		 Rubber-capped syringe, Sterile container, Transport Swab
Placenta Tissue or aspirate preferred; 2 swabs acceptable Rubber-capped syringe, Sterile container, Transport Swab
Bartholin gland Exudate, 0.5 mL Rubber-capped syringe or Transport Swab
Amniotic fluid Uncontaminated fluid?�10-15 mL preferred;?�8 mL
minimum		 Sterile container

Collection Instructions

Offsite Collection Instructions

See UCMC Collection Instructions

UCMC Collection Instructions

Specimen Source: Endometrium
Specimen Collection: Wipe cervix clean of vaginal secretions and mucus. Curetting or aspiration is preferred. If swabs are to be used, insertion through a sterile tube sheath will help avoid contamination with vaginal flora.

Specimen Transport: 24 hr, no refrigeration.


Specimen Source: Bartholin gland
Specimen Collection: Decontaminate skin with iodine. Aspirate abscess with needle and syringe or express exudate from duct. Aspirate is preferred.
Specimen Volume: 0.5 mL aspirate
Specimen Transport:  24 hr, no refrigeration.


Specimen Source: Amniotic fluid.

Anaerobic culture is recommended.
Specimen Collection: Pressure catheter, amniocentesis, or aspirations with syringe at Caesarean section.
Specimen Volume: 6-8 mL; 3 mL minimum.
Specimen Transport: 24 hr, no refrigeration.

Test Components

Gram stain

Aerobic culture

Methodology

Conventional culture media

Last Reviewed Date

08/12/2022

Additional Information

Genital Site Volume Transport
Endometrium Currettings or aspirate preferred; 2 swabs
acceptable		 Rubber-capped syringe, Sterile container, Transport Swab
Placenta Tissue or aspirate preferred; 2 swabs acceptable Rubber-capped syringe, Sterile container, Transport Swab
Bartholin gland Exudate, 0.5 mL Rubber-capped syringe or Transport Swab
Amniotic fluid Uncontaminated fluid?�10-15 mL preferred;?�8 mL
minimum		 Sterile container