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Test Code IGNC Culture, Genital, Female (Internal), Bacteria

Specimen and Container/Tube

Swab, fluid, or aspirate Site: Endometrium, placenta, Bartholin gland, amniotic fluid

Anaerobic culture is recommended for amniotic fluid. See Anaerobe/Aerobe culture (ANAC).

Transport swab, sterile container, or rubber-capped syringe. See ‘Additional Information’ for preferred volumes

Collection Instructions

Offsite Collection Instructions

See UCMC Collection Instructions

UCMC Collection Instructions

Specimen Source: Endometrium
Specimen Collection: Wipe cervix clean of vaginal secretions and mucus. Curetting or aspiration is preferred. If swabs are to be used, insertion through a sterile tube sheath will help avoid contamination with vaginal flora.

Specimen Transport: 24 hr, no refrigeration.


Specimen Source: Bartholin gland
Specimen Collection: Decontaminate skin with iodine. Aspirate abscess with needle and syringe or express exudate from duct. Aspirate is preferred.
Specimen Volume: 0.5 mL aspirate
Specimen Transport:  24 hr, no refrigeration.


Specimen Source: Amniotic fluid.

Anaerobic culture is recommended.
Specimen Collection: Pressure catheter, amniocentesis, or aspirations with syringe at Caesarean section.
Specimen Volume: 6-8 mL; 3 mL minimum.
Specimen Transport: 24 hr, no refrigeration.

Specimen Handling and Transport Instructions

Genital Site Volume Transport
Endometrium Currettings or aspirate preferred; 2 swabs
acceptable		 Rubber-capped syringe, Sterile container, Transport Swab
Placenta Tissue or aspirate preferred; 2 swabs acceptable Rubber-capped syringe, Sterile container, Transport Swab
Bartholin gland Exudate, 0.5 mL Rubber-capped syringe or Transport Swab
Amniotic fluid Uncontaminated fluid?�10-15 mL preferred;?�8 mL
minimum		 Sterile container

Rejection Criteria

Whole blood/unspun samples greater than 6 hours will be rejected.

Reference Values

Negative

Critical Values

 

All positive  culture

 

 

 

 

All

Test Components

Gram stain

Aerobic culture

CPT Codes

87070, 87205

LOINC Codes

30887070, 30687205

Methodology

Conventional culture media

Additional Information

Genital Site Volume Transport
Endometrium Currettings or aspirate preferred; 2 swabs
acceptable		 Rubber-capped syringe, Sterile container, Transport Swab
Placenta Tissue or aspirate preferred; 2 swabs acceptable Rubber-capped syringe, Sterile container, Transport Swab
Bartholin gland Exudate, 0.5 mL Rubber-capped syringe or Transport Swab
Amniotic fluid Uncontaminated fluid?�10-15 mL preferred;?�8 mL
minimum		 Sterile container

Volume

Genital Site Volume Transport
Endometrium Currettings or aspirate preferred; 2 swabs
acceptable		 Rubber-capped syringe, Sterile container, Transport Swab
Placenta Tissue or aspirate preferred; 2 swabs acceptable Rubber-capped syringe, Sterile container, Transport Swab
Bartholin gland Exudate, 0.5 mL Rubber-capped syringe or Transport Swab
Amniotic fluid Uncontaminated fluid?�10-15 mL preferred;?�8 mL
minimum		 Sterile container

Turnaround Time

Gram stain only: within 1 hr of receipt in Microbiology lab, if STAT requested.

STAT Turnaround Time

Gram stain only: within 1 hr of receipt in Microbiology lab, if STAT requested.

Day(s) Performed

24/7

STAT DAY(S) AND TIME(S) PERFORMED

Available

Last Reviewed Date

08/12/2022