Test Code HPVA High Risk HPV Probe Assay, Endocervical

Important Note

Random Collections ONLY.
NO preservatives or additives or sample will be rejected.

The Gen-Probe Aptima assay is a target amplification nucleic acid probe test that utilizes target capture, Transcription Mediated Amplification (TMA), and Hybridization Protection Assay (HPA) technologies for qualitative detection of Human Papillomavirus (HPV).

Test results may be affected by improper specimen collection, storage or specimen processing.

The Internal Control monitors the target capture, amplification and detection steps of the assay. It is not intended to control for cervical sampling adequacy.

A negative Aptima HPV Assay result does not exclude the possibility of cytologic abnormalities or of future or underlying CIN2, CIN3, or cancer

Infectious

Additional Codes

HPV
Human papillomavirus
Gen-Probe Aptima

Specimen and Container/Tube

Endocervical

PreservCyt ThinPrep Pap test vial

Specimen Handling and Transport Instructions

See UCMC collection instructions.

Sample in ThinPrep test vial can be stored at 2-8C for up to 30 days prior to testing.

Rejection Criteria

Random Collections ONLY.
NO preservatives or additives, or sample will be rejected.

Reference Values

Negative

Critical Values

All positive culture

All

Turnaround Time

Day(s) Performed

Testing is performed twice per week, on Tuesday and Friday.

STAT DAY(S) AND TIME(S) PERFORMED

Not Available

CPT Codes

87624

LOINC Codes

30687624

Volume

Specimen Minimum Volume

4 mL

Pediatric Volume

4 mL

Collection Instructions

Offsite Collection Instructions

See UCMC collection instructions

Sample in ThinPrep test vial can be stored at 2-8°C for up to 30 days prior to testing.

UCMC Collection Instructions

Specimen Source: Endocervical
Specimen Collection: Wipe cervix clean of vaginal secretions and mucus. Using a speculum with no lubricant, compress the cervix. Obtain exudate from endocervical glands. Please use the PreservCyt ThinPrep Pap test vials for all HPV tests, even when a cytologic exam is not requested. This test can be performed on the same sample collected for liquid-based ThinPrep Pap test.
Specimen Transport: Immediate, no refrigeration.

Microbiology Specimen Collection Tables
Please see link for Microbiology Specimen Collection Tables;

Category “Genital, Female, External”; Type "Cervix"Table 1 BACTERIOLOGY, CHLAMYDIA, MYCOLOGY, MYCOBACTERIOLOGY (AFB), VIROLOGY

Test Components

The assay detects high-risk HPV types 16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66, and 68 but does not discriminate among the types

Methodology