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Test Code HPFRP Helicobacter pylori with Clarithromycin Resistance Prediction, Molecular Detection, PCR, Feces


Ordering Guidance


Confirmation of eradication testing should not be ordered until 4 or more weeks after cessation of treatment.



Specimen Required


The high sensitivity of amplification by polymerase chain reaction requires the specimen to be processed in an environment in which contamination of the specimen by Helicobacter pylori DNA is unlikely.

 

Patient Preparation:

1. For 4 weeks prior to testing, patient should not take antibiotics.

2. For 2 weeks prior to testing, patient should not take proton pump inhibitors or bismuth compounds.

Supplies: Culture and Sensitivity Stool Transport Vial (T058)

Specimen Type: Preserved feces

Submission Container/Tube: Commercially available transport system specific for recovery of enteric pathogens from fecal specimens (15 mL of nonnutritive transport medium containing phenol red as a pH indicator, either Cary-Blair or Para-Pak C and S)

Specimen Volume: 5 mL

Collection Instructions:

1. Collect fresh fecal specimen and submit 1 gram or 5 mL in container with transport medium.

2. Place feces in preservative within 2 hours of collection.

3. Place vial in a sealed plastic bag and send ambient or refrigerated. Specimens sent frozen will be rejected.


Secondary ID

607594

Testing Algorithm

For more information see Helicobacter pylori Diagnostic Algorithm.

Method Name

Real-Time Polymerase Chain Reaction (PCR)

Specimen Type

Fecal

Specimen Minimum Volume

1 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Fecal Ambient (preferred) 7 days
  Refrigerated  7 days

Reject Due To

Fecal swab
ESwab transport medium
Feces in gel transport medium
ECOFIX preservative formalin
PVA fixative
Unpreserved stool
Reject

Reference Values

Not detected

Performing Laboratory

Mayo Clinic Laboratories in Rochester

CPT Code Information

87798

Day(s) Performed

Monday, Wednesday, Friday

Report Available

4 to 6 days

Forms

If not ordering electronically, complete, print, and send one of the following with the specimen:

1. Microbiology Test Request (T244)

2. Gastroenterology and Hepatology Test Request (T728)