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HelpTest Code FHGB Fetal hemoglobin
Specimen and Container/Tube
Specimen Type: Whole blood
Preferred Specimen: lavender top
Other Acceptable specimens: not applicable
Specimen Handling and Transport Instructions
UCMC Onsite Instructions: Collect specimen per standard collection procedure and send to laboratory immediately.
UCMC Offsite and UC MedLab Outreach Instructions: Collect specimen per standard collection procedure and send to laboratory immediately. Specimens must be received within 24 hours. If transport is delayed, store the sample in a refrigerator.
Rejection Criteria
- Common specimen rejected situations include: incorrect specimen type, insufficient volume, missing or incomplete specimen identifiers, incorrect specimen transport, or specimens outside stability limits.
- Frozen or heated specimens will be rejected
- Clotted or quantity not sufficient specimens are not suitable for testing and will be rejected
Reference Values
|
Analyte |
Age |
Sex |
Reference Range |
|
Percent fetal hemoglobin cells |
< 2 years |
Male and female |
>90.00% |
|
Percent fetal hemoglobin cells |
> 2 years |
Male and female |
< 0.10% |
|
F cells percent |
All |
Male and female |
< 5.8% |
|
Percent adult hemoglobin cells |
< 6 years |
Male and female |
< 10% |
|
Percent adult hemoglobin cells |
> 6 years |
Male and female |
> 90% |
Critical Values
not applicable
Turnaround Time
Turnaround times are relative to the time the specimen is received in the test laboratory.
STAT Turnaround Time: 60 minutes
Routine Turnaround Time: 24 hours
Testing Schedule: Monday - Friday 8:00 am - 5:00 pm and Saturday 8:00 am - 3:00 pm, if later specimens are expected the laboratory must be notified
CPT Codes
CPT codes
LOINC Codes
FHGB
Volume
Preferred Volume: 4.0 mL
Minimum Volume: 1.0 mL
Specimen Stability
|
Storage Temperature |
Stability |
|
Room temperature (20 - 25°C) |
24 hours |
|
Refrigerated (2 - 10°C) |
48 hours |
|
Frozen (-20°C) |
not acceptable |
Collection Instructions
Collect specimen per standard laboratory collection procedures.
Test Components
Flow Cytometry: Percentage of fetal cells, "F' cells, and adult cells in maternal blood specimen
Kleihauer Betke: Percentage of fetal cells and adult cells in maternal blood specimen
Clinical Indications
- Fetal maternal hemorrhage
Methodology
Flow cytometry or Kleihauer-Betke acid slide elution method.
Kleihauer Betke Method will be performed outside of flow cytometry hours for STAT orders. Routine orders are held for flow cytometry method performed Monday through Saturday 8:00am - 4:30pm.
Synonyms/Search Keywords
KBT, fetal-maternal hemorrhage
Last Reviewed Date
9/5/2024