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The University of Chicago Medicine Laboratories

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Specimen Collection and Handling

Transfusion

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Mayo Clinic Laboratories

Test Code FENTU Fentanyl, Qualitative Screen, Urine

Specimen and Container/Tube

Specimen Type: Urine, Random Collection

Preferred Specimen: Sterile Plastic Container

Other Acceptable specimens: Sterile Clean Glass Container

Volume

Preferred Volume: 3.0 mL

Minimum Volume: 1.0 mL

Specimen Stability

Storage Temperature

Stability

Room temperature

4 weeks

Refrigerated

4 weeks

Frozen

6 months

Collection Instructions

  • Collect specimen per standard laboratory collection procedures. 
  • No preservatives or additives.

Specimen Handling and Transport Instructions

UCMC Onsite Instructions: 

  • Collect specimen per standard collection procedure and send to laboratory immediately. 
  • No preservatives or additives.

 

UCMC Offsite and UC MedLab Outreach Instructions: 

  • Samples should be centrifuged within 2 hours of collection
  • No preservatives or additives
  • Centrifuge specimens at >2500 x g for 10 minutes at room temperature.
  • Aliquot urine into plastic tube containing a minimum of 0.2 mL of specimen.
  • Urine samples must then be sent refrigerated at 2-8oC.

Rejection Criteria

  • Common specimen rejected situations include: incorrect specimen type, insufficient volume, missing or incomplete specimen identifiers, incorrect specimen transport, or specimens outside stability limits. 
  • Specimens that are collected with preservatives or additives.

Reference Values

Test

Age

Sex

Reference Range

Fentanyl

All

All

None Detected

Critical Values

Analyte

Unit

Low

High

Age

N/A

N/A

N/A

N/A

N/A

Turnaround Time

Turnaround times are relative to the time the specimen is received in the test laboratory. 

 

STAT Turnaround Time: 1 hour

Routine Turnaround Time: 4 hours

Testing Schedule: 24/7

Clinical Indications

  • For medical purposes only. 
  • Qualitative screening for norfentanyl in urine.
  • The cutoff for the assay is 5 ng/mL norfentanyl.
  • This test must not be used for employee drug testing or other purposes requiring a chain of custody handling procedure.

Test Components

Fentanyl Screen

Methodology

Homogeneous Enzyme Immunoassay (Roche Diagnostics)

CPT Codes

80307

LOINC Codes

19270-8

Synonyms/Keyword Search

DAT: Drugs of Abuse Testing, FENTU, fentanyl, norfentanyl

Additional Information

  • Results should always be assessed in conjunction with patient's medical history, clinical examination and other findings.
  • Also included in urine toxicology screen; EDS order code.
  • The Emergency Drug Screen (EDS) includes screening tests for Ethanol, Amphetamines, Barbiturates, Benzodiazepines, Cocaine Metabolite, Opiates, Phencyclidine, Methadone Metabolite, Oxycodone and Fentanyl Metabolite.
  • All positive results are considered presumptive unless confirmed by an alternate method.
  • Cutoff values used to discern positive from None Detected: 5 ng/mL or greater. The concentration of drug detected cannot be estimated by this qualtitaive method.

Last Review Date

8/19/2024