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Test Code DHVD 1,25-Dihydroxyvitamin D, Serum

Important Note

Use Sunquest code VD125

Synonym "VD125"

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Specimen Type

Serum


Advisory Information


The 25-hydroxyvitamin D test (25HDN / 25-Hydroxyvitamin D2 and D3, Serum) in serum the preferred initial test for assessing vitamin D status and most accurately reflects the body's vitamin D stores. In the presence of renal disease or hypercalcemia, testing of 1,25-dihydroxy vitamin D (DHVD) might be needed to adequately assess vitamin D status.



Specimen Required


Patient Preparation: Fasting (4-hour preferred but not required)

Container/Tube:

Preferred: Red top

Acceptable: Serum gel

Specimen Volume: At least 1.5 mL


Specimen Minimum Volume

1.2 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Serum Refrigerated (preferred) 7 days
  Frozen  28 days
  Ambient  7 days

Reference Values

Males:

<16 years: 24-86 pg/mL

≥16 years: 18-64 pg/mL

 

Females:

<16 years: 24-86 pg/mL

≥16 years: 18-78 pg/mL

 

For SI unit Reference Values, see https://www.mayocliniclabs.com/order-tests/si-unit-conversion.html

Day(s) and Time(s) Performed

Monday through Friday; 3 p.m.

CPT Code Information

82652

Reject Due To

Gross hemolysis Reject
Gross lipemia OK
Gross icterus OK

Method Name

Extraction/Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)

Forms

If not ordering electronically, complete, print, and send 1 of the following forms with the specimen:

-General Request (T239)

-Renal Diagnostics Test Request (T830)