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Test Code DHRP Dihydrorhodamine Flow Cytometric Phorbol Myristate Acetate Test, Blood

Important Note

Order LABRFDHRP in EPIC 

Specimen Type

WB Sodium Heparin


Shipping Instructions


Testing is not performed on Saturday, Sunday, or observed holidays. Only collect and ship specimens for arrival on days when testing is performed.

 

Specimens received on days when testing is not performed or after 5 p.m. Central on Friday will be canceled if specimen is outside of stability when testing is next performed.

 

Collect and package specimen as close to shipping time as possible. Ship specimen overnight in an Ambient Shipping Box-Critical Specimens Only (T668) following the instructions in the box. It is recommended that specimens arrive within 24 hours of collection.



Necessary Information


Ordering healthcare professional name and phone number are required.



Specimen Required


Two whole-blood sodium heparin specimens are required, one from the testing patient and the other from an unrelated healthy donor as a control.

 

Supplies: Ambient Shipping Box-Critical Specimens Only (T668)

 

Patient:

Container/Tube: Green top (sodium heparin)

Specimen Volume: 5 mL

Collection Instructions: Send whole blood specimen in original tube. Do not aliquot.

 

Normal Control:

Container/Tube: Green top (sodium heparin)

Specimen Volume: 5 mL

Collection Instructions:

1. Collect a control specimen from the unrelated healthy donor within an hour of the patient's specimen collection time.

2. Label clearly as Normal Control and the corresponding patient information.

3. Send the whole blood specimen in the original tube. Do not aliquot.

4. Rubber band patient specimen and normal control vials together.


Specimen Minimum Volume

1 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
WB Sodium Heparin Ambient 48 hours GREEN TOP/HEP

Reject Due To

Gross hemolysis Reject
Gross lipemia Reject

Reference Values

Result Name

Unit

Cutoff for defining normal

% PMA ox-DHR+

%

≥95%

MFI PMA ox-DHR+

MFI

≥60

Control % PMA ox-DHR+

%

≥95%

Control MFI PMA ox-DHR+

MFI

≥60

 

MFI = mean fluorescence intensity

PMA = phorbol myristate acetate

DHR = dihydrorhodamine

 

The appropriate age-related reference values for Absolute Neutrophil Count will be provided on the report.

Day(s) Performed

Monday through Friday

Report Available

3 to 4 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

CPT Code Information

86352

Method Name

Flow Cytometry

Secondary ID

62765