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Test Code CCSF Cerebrospinal Fluid Cell Count

Specimen and Container/Tube

Specimen Type: Cerebrospinal fluid

Preferred Specimen: sterile container

Other Acceptable specimens: not applicable

Specimen Handling and Transport Instructions

 

UCMC Onsite Instructions: Collect specimen per standard collection procedure and send to laboratory immediately.

 

UCMC Offsite and UC MedLab Outreach Instructions: Collect specimen per standard collection procedure and send to laboratory immediately. Specimens must be received within 2 hours. If transport is delayed, refrigerate specimens prior to transport. 

Rejection Criteria

  • Common specimen rejected situations include: incorrect specimen type, insufficient volume, missing or incomplete specimen identifiers, incorrect specimen transport, or specimens outside stability limits. 
  • Frozen or heated specimens will be rejected
  • Quantity not sufficient specimens are not suitable for testing and will be rejected 
  • Clotted specimen are not suitable for testing. Testing may be attempted, but not all results may be reported. 

Reference Values

Analyte

Age

Sex

Reference Range

Nucleated cells CSF

12 years and up

Male and female

0 – 5 /uL

Nucleated cells CSF

4 years

Male and female

0 – 10 /uL

Nucleated cells CSF

1 year

Male and female

0 – 20 /uL

Nucleated cells CSF

0 days

Male and female

0 – 30 /uL

RBC CSF

All

Male and female

0 /uL

CSF neutrophil percent

2 months

Male and female

0 – 6 %

CSF neutrophil percent

0 days

Male and female

0 – 8 %

CSF lymphocyte percent

2 months

Male and female

40 – 80 %

CSF lymphocyte percent

0 days

Male and female

5 -35 %

CSF monocyte percent

2 months

Male and female

15 – 45 %

CSF monocyte percent

0 days

Male and female

50 – 90 %

CSF eosinophil percent

All

Male and female

0 %

CSF basophil percent

All

Male and female

0 %

Critical Values

Analyte

Unit

Low

High

Age

Intracellular organisms and intracellular or extracellular parasites in: blood, CSF, pericardial fluid, peritoneal fluid (paracentesis), pleural fluid, synovial fluid; intracellular and extracellular yeast and pneumocystis in BAL.

Turnaround Time

Turnaround times are relative to the time the specimen is received in the test laboratory. 

 

STAT Turnaround Time: 120 minutes

 

Routine Turnaround Time: 480 minutes

 

Testing Schedule: 24/7

CPT Codes

89051

LOINC Codes

CAPP 10333-3, CTOT 19075-1, CWBC 26465-5, CRBC 792-2, CNEU 26512-4, CEOS 12208-5, CLYM 10328-3, CMON 10329-1, CBAS 13519-4, CPLA 51385-3

 

Volume

Preferred Volume: 3.0 mL

Minimum Volume: 0.5 mL

Specimen Stability

Storage Temperature

Stability

Room temperature

(20 - 25°C)

2 hours 

Refrigerated

(2 - 10°C)

2 hours 

Frozen

(-20°C)

not acceptable

Collection Instructions

Collect specimen per standard laboratory collection procedures. 

Test Components

WBC, RBC, and total cell counts, WBC differential, pathologist interpretation if requested 

Clinical Indications

  • Information concerning infection disease processes, cerebral hemorrhage, malignant diseases, etc. may be obtained from the cell count and differential.

Methodology

Glocyte Automated CSF cell counter and manual hemocytometer method, Wright stain and light microscopy 

Synonyms/Search Keywords

CSF count, cell count CSF

Additional Information

Not applicable 

Last Reviewed Date

8/12/2024