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Test Code BNP NT-proBNP

Important Note

This test may be affected by biotin interference. Patients on high dose biotin (5mg/day or more) should not have samples drawn until at least 24 hours after the last biotin administration.

Specimen and Container/Tube

Specimen Type: Plasma

Preferred Specimen: Li-Heparin Mint Green Top

Other Acceptable specimens: Serum/Gold Top, Serum/Red Top

Specimen Handling and Transport Instructions

UCMC Onsite Instructions: 

  • Collect specimen per standard collection procedure and send to laboratory immediately. 

 

UCMC Offsite and UC MedLab Outreach Instructions: 

  • Samples should be centrifuged within 2 hours of collection.
  • Centrifuge specimens at >2500 x g for 10 minutes at room temperature.
  • Aliquot sample into plastic transport tube containing a minimum of 0.2 mL of specimen.
  • Samples must be sent room temperature at 15-25oC.

Rejection Criteria

  • Common specimen rejected situations include: incorrect specimen type, insufficient volume, missing or incomplete specimen identifiers, incorrect specimen transport, or specimens outside stability limits. 
  • Specimens that are clotted or grossly hemolyzed.
  • Specimens that were transported unspun/whole blood and draw time is greater than 6 hours

Reference Values

Test

Age

Sex

Reference Range

NT-proBNP

<75 years

All

<125 pg/mL

NT-proBNP

>75 years

All

<450 pg/mL

*NOTE: Reference ranges are based on in-house studies performed at the University of Chicago Medicine by the Clinical Chemistry Laboratories per the Roche cobas 8000 modular series e801 System Installation Workbook  and the e801 PBNP product insert.

Critical Values

Analyte

Unit

Low

High

Age

N/A

N/A

N/A

N/A

N/A

CPT Codes

83880

LOINC Codes

30934-4

Volume

Preferred Volume: 0.5 mL

Minimum Volume: 0.2 mL

Specimen Stability

Storage Temperature

Stability

Room Temperature

3 days

Refrigerated

6 days

Frozen

24 months

Collection Instructions

Collect specimen per standard laboratory collection procedures. 

 

Test Components

N-Terminal Pro-B Type Natriuretic Peptide

Clinical Indications

Used as an aid in the diagnosis of individuals suspected of having congestive heart failure. The test is further indicated for the risk stratification of patients with acute coronary syndrome and congestive heart failure. The test may also serve as an aid in the assessment of increased risk of cardiovascular events and mortality in patients at risk for heart failure who have stable coronary artery disease.

Methodology

Electrochemiluminescence immunoassay (ECLIA) (Roche Diagnostics)

Additional Information

Results should always be assessed in conjunction with patient's medical history, clinical examination and other
findings.

 

Once per admit. If serial monitoring needed, Q daily.

Turnaround Time

Turnaround times are relative to the time the specimen is received in the test laboratory. 

 

STAT Turnaround Time: 1 hour

Routine Turnaround Time: up to 4 hours

Testing Schedule:  24/7

Synonyms/Keyword Search

PBNP, ProBNP

Last Review Date

8/19/2024