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Test Code ABO2ND ABO Verification

Important Note

  • This is a one-time test indicated only for patients with a Type and Screen order who lack a previous blood type on file at UCM.
  • This specimen must be collected in a separate draw from the Type and Screen sample; both samples must have separate collection times.
    • If collected from the patient by venipuncture, the patient MUST undergo an additional venipuncture procedure for the collection of this sample.
    • If collected from a line, the line must be accessed an additional and sepearate time for the collection of this sample.
    • The sample DOES NOT have to be collected on the same day as the type and screen.
  • Having a second person positively ID the patient and collect this sample (a different person from the one that collected the type and screen) is not required but is ideal and provides the greatest amount of patient safety.
  • Group O emergency uncrossmatched blood may be provided if this test is indicated, not yet completed, and the patient's condition requires transfusion before testing can be completed.

Specimen and Container/Tube

Whole Blood

Pink Top (EDTA)

  • Must be a separate "Collection Event" from the collection of the type and screen.
    • If collected by venipuncture, the patient must be stuck twice.
    • If collected from a line, the line must be accessed two different times.
  • MUST be a completely separate draw from the Type and
    Screen sample (documented collection time CANNOT be the same)

  • NOTE: All blood bank samples must have HAND WRITTEN collection information on the label to be accepted (date MM/DD/YY or MM/DD/YYYY, time, and initials). 

     

  • If the ABO2ND has already been completed when the current Type and
    Screen is needed – it does not need to be repeated
  • If the patient already has an ABORh on file with UCM Blood Bank
    when ordering a type and screen, an ABO2ND is not required

Rejection Criteria

This sample will be rejected if not labeled properly:

  • Must be labeled with patient LAST name, FIRST name and MRN
  • All blood bank samples must have HAND WRITTEN collection information on the label to be accepted (date MM/DD/YY or MM/DD/YYYY, time, and initials).
  • All information must be legible.

Turnaround Time

15 min

Request for emergent / life threatening / MTP should be
initiated by a phone call to Blood Bank at 2-6827.

Preferred Volume

2 mL

Pediatric Volume

0.5 mL

Additional Information

  • This is a one-time test indicated only for patients with a
    Type and Screen order who lack a previous blood type on file at
    UCM.
  • This specimen must be collected in a separate draw from the
    Type and Screen sample; both specimens must have separate
    collections times.
  • Group O blood will be provided in an emergency or for
    pediatricpatients if this test is indicated and not yet
    completed.
  • If there is a need for a transfusion, at least two blood
    types from at least two separate blood collection events must be
    on file.
  • Does not necessarily have to be done on the same day
    but before a type specific transfusion can take place, the
    requirement is to have 2 blood types from different blood
    collection events on file.

Transport Instructions

Deliver sample directly to blood bank, CCD 2-730 or via PTS Tube Station 400.  Blood bank samples sent to lab receiving first will be delayed a minimum of 30 additional minutes.

Special Instructions

Ordering:

  • Inpatient:  This test is ordered as needed by UCM Blood Bank
  • Outpatient:  This test is available for ordering by Clinical Providers

Last reviewed

Last reviewed 08/07/2024 RM