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HelpTest Code HBSAG Hepatitis B Surface Antigen
Infectious
Specimen and Container/Tube
Specimen Type: Serum
Preferred Specimen: Red Top
Specimen Handling and Transport Instructions
UCMC Onsite Instructions:
Collect specimen per standard collection procedure and send to laboratory immediately.
UCMC Offsite and UC MedLab Outreach Instructions:
- Samples should be centrifuged within 2 hours of collection.
- Centrifuge specimens at >2500 x g for 10 minutes at room temperature.
- Aliquot sample into plastic transport tube containing a minimum of 0.2 mL of specimen.
- Samples must be sent room temperature at 15-25oC.
Rejection Criteria
- Common specimen rejected situations include: incorrect specimen type, insufficient volume, missing or incomplete specimen identifiers, incorrect specimen transport, or specimens outside stability limits.
- Specimens that are clotted or grossly hemolyzed.
- Specimens that were transported unspun/whole blood and draw time is greater than 6 hours
Reference Values
This is a qualitative test.
|
Test |
Age |
Sex |
Reference Range |
Interpretation |
|
HBsAg |
All |
All |
<0.9 COI |
Non-Reactive |
|
HBsAg |
All |
All |
≥0.9<1.0 COI |
Borderline |
|
HBsAg |
All |
All |
≥1.0 |
Reactive |
Critical Values
|
Analyte |
Unit |
Low |
High |
Age |
|
N/A |
N/A |
N/A |
N/A |
N/A |
CPT Codes
87340
87341 (if appropriate)
LOINC Codes
5196-1
Volume
Preferred Volume: 0.5 mL
Minimum Volume: 0.2 mL
Specimen Stability
|
Storage Temperature |
Stability |
|
Room Temperature |
6 days |
|
Refrigerated |
14 days |
|
Frozen |
6 months |
Note: May be frozen up to 6 times.
Collection Instructions
Collect specimen per standard laboratory collection procedures.
Test Components
Hepatitis B surface Antigen
Clinical Indications
Hepatitis B virus (HBV) is a DNA virus that is endemic throughout the world. The infection is spread primarily through percutaneous contact with infected blood products (eg, blood transfusion, sharing of needles among injection drug users). The virus is also found in various human body fluids, and it is known to be spread through oral and genital contact. HBV can be transmitted from mother to child during delivery through contact with blood and vaginal secretions, but it is not commonly transmitted transplacentally.
HBV surface antigen (HBsAg) is the first serologic marker appearing in the serum at 6 to 8 weeks following exposure to HBV. In acute infection, HBsAg usually disappears in 1 to 2 months after the onset of symptoms. Persistence of HBsAg for more than 6 months in duration indicates development of either a chronic carrier state or chronic HBV infection
Methodology
Electrochemiluminescence immunoassay (ECLIA) (Roche Diagnostics)
Additional Information
A reactive screen result (cutoff index value >1.00) confirmed as positive by hepatitis B surface antigen (HBsAg) confirmatory test is indicative of acute or chronic hepatitis B, or chronic hepatitis B virus (HBV) carrier state.
Specimens with initially reactive screen results, but negative (not confirmed) by HBsAg confirmatory test results, are likely to contain cross-reactive antibodies from other infectious or immunologic disorders. These unconfirmed HBsAg-reactive screening test results should be interpreted in conjunction with test results of other HBV serologic markers (eg, HBs antibody; hepatitis B core [HBc] total antibody, and HBc IgM antibody). If clinically indicated, repeat testing at a later date is recommended.
Confirmed presence of HBsAg is frequently associated with HBV replication and infectivity, especially when accompanied by presence of hepatitis B e antigen or detectable HBV DNA.
Turnaround Time
Turnaround times are relative to the time the specimen is received in the test laboratory.
STAT Turnaround Time: Not available as STAT test
Routine Turnaround Time: up to 4 hours
Testing Schedule: 24/7
Synonyms/Keyword Search
Hepatitis Bs Ag
HBsAg (Hepatitis Bs Antigen)
HBV surface antigen
HBV s antigen
Hepatitis B surface antigen
Hepatitis Bs antigen
HBAG
Last Review Date
06/14/2024