Test Code VWDPKG von Willebrand Disease Panel
Specimen Type
Plasma
Container/Tube
Three light blue top tubes, 3.2% sodium citrate
Offsite Collection Instructions
Whole blood sample must arrive in the laboratory within
three hours of specimen collection.
If transport time exceeds three hours, follow centrifugation
instructions:
- Samples must be centrifuged at >2500 x g for 10 minutes
at room temperature. - Carefully remove the plasma from the cells using a plastic
transfer pipette avoiding the buffy coat. - Aliquot removed plasma into 6 plastic tubes containing a
minimum of 500 uL of specimen in each tube. - The plasma samples must then be frozen at -20 C.
- Once solidly frozen, they must be shipped on dry ice.
Samples that have thawed in transit will be rejected.
Note: For clients who do not have access to dry ice DO
NOT freeze the specimen. Follow the centrifugation instructions
and
send fresh plasma or send whole blood sample. It now becomes
vital that the specimen arrives in the lab within three hours
of
collection.
Turnaround Time
3-4 days
Test Includes
Factor VIII Coagulant Activity (FA8),
VWF Antigen Level (VWAG).
VWF GPIB Binding Actinity (VWFF).
VWF Collagen Binding Activity (VWFCBA).
Pathologist interpretation of abnormal results.
Note: von Willebrand Factor Multimeric Analysis will also
be
included if required by pathologist for diagnosis.
Preferred Volume
3 mL in 6 aliquots, 0.5 mL each
UCMC Collection Instructions
Specimens may be collected and sent to the laboratory at any
time.
Tests can be added on to an existing order up to 4 hours
following
the time of blood draw.
Additional Information
This package currently assesses both von Willebrand Factor
(vWF) antigenic level and functional attributes. Functional
attributes include an assessment of vWF interaction with
platelets
(specific antibody directed against the platelet binding site of
vWF), interaction
with collagen (assayed as collagen binding activity), and
interaction
with factor VIII (assayed as circulating factor VIII activity,
each of which is also orderable individually if preferred.
If required for diagnosis by the pathologist, send-out testing
for Multimeric Analysis will also be performed. As
appropriate, recommendations for further specialized testing
such as ristocetin-induced platelet aggregation or DNA analysis
may
also be made in individual cases, but the performance of such
testing will require additional orders (and in the case of
ristocetin-induced platelet aggregation, a separate blood draw
on the day of testing).
All 3.2% sodium citrate light blue top tubes require a ratio
of 9 parts blood to 1 part anticoagulant (example: a 3.0 mL
tube
requires 2.7 mL blood added to 0.3 mL of
anticoagulant).
Fee Code
3058520, 30585246, 30585245, 33183520
Synonyms
vWD Panel, von Willebrand Factor, vWF, vWF Disease
Test Usage
Diagnosis and management of von Willebrand disease
STAT Turnaround Time
n/a
Specimen Minimum Volume
3 mL in 6 aliquots, 0.5 mL each
DAY(S) AND TIME(S) PERFORMED
Factor VIII Coagulant Activity (FA8). Test performed 7days
a week from 8:00 am to 10:30 pm. Samples received in the
laboratory after 10:30 pm will be run the next morning.
VWF Antigen Level (VWAG): Test performed 7 days a week
from 8:00 am to 10:30 pm. Samples received in the laboratory
after
10:30 pm will be run the next morning.
VWF GPIB Binding Actinity (VWFF): Test performrd
Monday - Friday, 8:00 am to 2:00 pm.
VWF Collagen Binding Activity (VWFCBA):
Test performed twice a week (Tuesdays and Thursdays).
Sample must be received in the lab
before 9:00 am to be included in the run.
Pathologist interpretation of abnormal results.
Note: von Willebrand Factor Multimeric Analysis will also
be included if required by pathologist for diagnosis.
STAT DAY(S) AND TIME(S) PERFORMED
Not Available
CPT
85240, 85246, 85245, 83520
Method Name
Factor VIII Coagulant Activity (FA8): Clotting
VWF Antigen Level (VWAG): Immunoturbidimetric (latex
immunoassay)
VWF GPIB Binding Actinity (VWFF): Immunoturbidimetric (latex
immunoassay)
VWF Collagen Binding Activity (VWFCBA): ELISA
Reference Values
Factor VIII Coagulant Activity (FA8): 57-152%
VWF Antigen Level (VWAG): 55-200%
VWF GPIB Binding Actinity (VWFF): 50-155%
VWF Collagen Binding Activity (VWFCBA): 46-159%
Reject Due To
Specimens that have wrong ratio of blood to anticoagulant
(fill volume) clotted or grossly hemolyzed.