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Test Code VWDPKG von Willebrand Disease Panel

Specimen Type

Plasma

Container/Tube

Three light blue top tubes, 3.2% sodium citrate

Offsite Collection Instructions

Whole blood sample must arrive in the laboratory within
three hours of specimen collection.

 

If transport time exceeds three hours, follow centrifugation
instructions:

  • Samples must be centrifuged at >2500 x g for 10 minutes
    at room temperature.
  • Carefully remove the plasma from the cells using a plastic
    transfer pipette avoiding the buffy coat.
  • Aliquot removed plasma into 6 plastic tubes containing a
    minimum of 500 uL of specimen in each tube.
  • The plasma samples must then be frozen at -20 C.
  • Once solidly frozen, they must be shipped on dry ice.
    Samples that have thawed in transit will be rejected.

Note: For clients who do not have access to dry ice DO
NOT freeze the specimen. Follow the centrifugation instructions and
send fresh plasma or send whole blood sample. It now becomes
vital that the specimen arrives in the lab within three hours of
collection.

Turnaround Time

3-4 days

Test Includes

Factor VIII Coagulant Activity (FA8),
VWF Antigen Level  (VWAG).
VWF GPIB Binding Actinity (VWFF).
VWF Collagen Binding Activity (VWFCBA).
Pathologist interpretation of abnormal results.
Note: von Willebrand Factor Multimeric Analysis will also
be
included if required by pathologist for diagnosis.

Preferred Volume

3 mL in 6 aliquots, 0.5 mL each

UCMC Collection Instructions

Specimens may be collected and sent to the laboratory at any
time.
Tests can be added on to an existing order up to 4 hours
following
the time of blood draw.

Additional Information

This package currently assesses both von Willebrand Factor
(vWF) antigenic level and functional attributes. Functional
attributes include an assessment of vWF interaction with platelets
(specific antibody directed against the platelet binding site of vWF), interaction
with collagen (assayed as collagen binding activity), and interaction
with factor VIII (assayed as circulating factor VIII activity,
each of which is also orderable individually if preferred.
If required for diagnosis by the pathologist, send-out testing
for Multimeric Analysis will also be performed. As
appropriate, recommendations for further specialized testing
such as ristocetin-induced platelet aggregation or DNA analysis may
also be made in individual cases, but the performance of such
testing will require additional orders (and in the case of
ristocetin-induced platelet aggregation, a separate blood draw
on the day of testing).

All 3.2% sodium citrate light blue top tubes require a ratio
of 9 parts blood to 1 part anticoagulant (example: a 3.0 mL tube
requires 2.7 mL blood added to 0.3 mL of
anticoagulant).

Fee Code

3058520, 30585246, 30585245, 33183520

Synonyms

vWD Panel, von Willebrand Factor, vWF, vWF Disease

Test Usage

Diagnosis and management of von Willebrand disease

STAT Turnaround Time

n/a

Specimen Minimum Volume

3 mL in 6 aliquots, 0.5 mL each

DAY(S) AND TIME(S) PERFORMED

Factor VIII Coagulant Activity (FA8). Test performed 7days

a week from 8:00 am to 10:30 pm. Samples received in the
laboratory after 10:30 pm will be run the next morning.


VWF Antigen Level  (VWAG): Test performed 7 days a week
from 8:00 am to 10:30 pm. Samples received in the laboratory after
10:30 pm will be run the next morning.


VWF GPIB Binding Actinity (VWFF): Test performrd
Monday - Friday, 8:00 am to 2:00 pm.


VWF Collagen Binding Activity (VWFCBA):
Test performed twice a week (Tuesdays and Thursdays).

Sample must be received in the lab
before 9:00 am to be included in the run.


Pathologist interpretation of abnormal results.
Note: von Willebrand Factor Multimeric Analysis will also
be included if required by pathologist for diagnosis.

STAT DAY(S) AND TIME(S) PERFORMED

Not Available

CPT

85240, 85246, 85245, 83520

Method Name

Factor VIII Coagulant Activity (FA8): Clotting
VWF Antigen Level (VWAG): Immunoturbidimetric (latex immunoassay)
VWF GPIB Binding Actinity (VWFF): Immunoturbidimetric (latex immunoassay)
VWF Collagen Binding Activity (VWFCBA): ELISA

Reference Values

Factor VIII Coagulant Activity (FA8): 57-152%
VWF Antigen Level (VWAG): 55-200%
VWF GPIB Binding Actinity (VWFF): 50-155%
VWF Collagen Binding Activity (VWFCBA): 46-159%

Reject Due To

Specimens that have wrong ratio of blood to anticoagulant
(fill volume) clotted or grossly hemolyzed.