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HelpTest Code URAM Urine Amylase, 24 hour
Specimen and Container/Tube
Urine, Timed (24 hour)
Rejection Criteria
Urine containing chelating agents (citrates, oxalates,
EDTA) must not
be used.
Turnaround Time
N/A
Test Includes
Quantitative determination of amylase in urine
Specimen Type
Urine, Timed (24 hour)
Container/Tube
Sterile Urine Container
Preferred Volume
0.5 mL
Specimen Minimum Volume
0.2 mL
Reject Due To
Urine containing chelating agents (citrates, oxalates,
EDTA) must not
be used.
Day(s) Performed
Monday – Friday
STAT DAY(S) AND TIME(S) PERFORMED
N/A
STAT Turnaround Time
N/A
Method Name
Enzymatic Colorimetric Assay
Spectrophotometric
Synonyms
N/A
Test Limitations
a- Amylase is unstable in acid urine. Assay promptly or
adjust
pH to alkaline range (about pH 7) before storage. Freezing has
caused substantial loss of amylase activity in some urine
specimens
and is therefore not recommended.
Test Usage
Assist in the assessment of liver synthetic function.
Useful in the diagnosis and management of pancreatitis. An aid
in
the evaluation of pancreatic function.
Offsite Collection Instructions
1. Follow clinic instructions for 24 hour collection
process.
2. Keep urine refrigerated during the 24 hour collection
process.
3. Aliquot 10 mL from a well mixed timed sample collection.
4. Indicate collection volume and duration.
5. Transport refrigerated (2-8 ° C).
UCMC Collection Instructions
1. Follow clinic instructions for 24 hour collection
process.
2. Keep urine refrigerated during the 24 hour collection
process.
3. Aliquot 10 mL from a well mixed timed sample collection.
4. Indicate collection volume and duration.
5. Transport refrigerated (2-8 ° C).
Reference Values
<400 IU/L
(This value is NOT
calculated for total
volume)
Additional Information
N/A
Stability: Room Temp
2 days
Stability: Refrigerated
10 days
Stability: Frozen
n/a
Pediatric Volume
0.2 mL
Clinical Indications
Assist in the assessment of liver synthetic function.
Useful in the diagnosis and management of pancreatitis. An aid
in
the evaluation of pancreatic function.
Transport Instructions
1. Follow clinic instructions for 24 hour collection
process.
2. Keep urine refrigerated during the 24 hour collection
process.
3. Aliquot 10 mL from a well mixed timed sample collection.
4. Indicate collection volume and duration.
5. Transport refrigerated (2-8 ?� C).
Test Components
Quantitative determination of amylase in?�urine
Specimen Stability
2 days
Methodology
Enzymatic Colorimetric Assay
Spectrophotometric
Synonyms/Key Search Words
N/A